| It is our pleasure to inform you that your remarkable article has been cited in JPC - Journal of Planar Chromatography - Modern TLC published by Akadémiai Kiadó. Through our Cited Author's Alert service we hope we can provide you some relevant information and make you aware of JPC - Journal of Planar Chromatography - Modern TLC, a publication in your research area. Your article: Ethnomedicines used in Trinidad and Tobago for urinary problems and diabetes mellitus Lans, CA JOURNAL OF ETHNOBIOLOGY AND ETHNOMEDICINE 115 (2006) Has been cited in:Multiple marker-based evaluation of Kalanchoe pinnata, Bombax ceiba, and Morus alba leaves: Quantification of alpha-amyrin, lupeol, and beta-sitosterol using high-performance thin-layer chromatography Hemendra Yadav, Mukesh Kumar, Manish Nivsarkar, Sheetal Anandjiwala JPC - Journal of Planar Chromatography - Modern TLC Volume 27, Number 6; December 2014, pp. 438-443. |
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PDF (474.6 KB)Authors
Hemendra Yadav1, Mukesh Kumar1, Manish Nivsarkar2, Sheetal Anandjiwala1 
1National Institute of Pharmaceutical Education and
Research — Ahmedabad Department of Natural Products S. G. Highway
Thaltej, Ahmedabad 380054 Gujarat India
2B. V. Patel Pharmaceutical Education and Research Development (PERD) Centre Department of Pharmacology and Toxicology S. G. Highway Thaltej, Ahmedabad 380054 Gujarat India
Abstract
Kalanchoe pinnata (Crassulaceae), Bombax ceiba (Bombaceae), and Morus alba (Moraceae) are important drugs in Indian and Chinese Systems of Medicine. Leaves of K. pinnata
are reported to show anti-inflammatory, antifungal, and antibacterial
activity and are reported to contain quercetin, kaempferol, α-amyrin,
β-sitosterol, ferulic, and caffeic acid. Leaves of B. ceiba are
reported for the treatment of diarrhea, dysentery, gonorrhea, and
bladder ulceration and are reported to contain shamimin, lupeol,
β-sitosterol, and α-amyrin. Leaves of M. alba are reported to
show anti-inflammatory, emollient, hypolipidemic, and diuretic activity
and are reported to contain quercetin, rutin, moracetin, and artocarpin.
In the present work, a method for simultaneous quantification of three
marker compounds, viz., α-amyrin, lupeol, and β-sitosterol from the
leaves of K. pinnata, B. ceiba, and M. alba was
developed and validated as per International Conference on Harmonization
(ICH) guidelines. This is the first report of simultaneous
quantification of these three compounds. α-Amyrin, lupeol, and
β-sitosterol resolved at RF 0.71, 0.28, and 0.16,
respectively, on thin-layer chromatography (TLC) when developed in the
solvent system of toluene-ethyl acetate (9.5:0.5). The linearity range
for α-amyrin, lupeol, and β-sitosterol was found to be 160–560, 150–900,
and 80–480 ng spot−1, respectively, with correlation coefficients (r
values) of 0.999, 0.997, and 0.995, respectively. The amount of
α-amyrin, lupeol, and β-sitosterol were found to be in range of
0.013–0.047, 0.046–0.318, and 0.028–0.123 % w/w,
respectively. The developed methods were found to be accurate, precise,
and reproducible and can be used for routine quality control of herbal
material and formulations containing K. pinnata, B. ceiba, and M. alba.
Keywords
Kalanchoe pinnata, Bombax ceiba, Morus alba, High-performance thin-layer chromatography, α-Amyrin, Lupeol, β-Sitosterol
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References
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S. Anandjiwala, H. Srinivasa, M. Rajani, Chromatographia 66 (2007) 725–734.
[CrossRef]
[CrossRef] N.P. Yadav, V.K. Dixit, J. Ethnopharmacol. 86 (2003) 197–202.
[CrossRef] A.P. Almeida, S.A. Da Silva, M.L. Souza, L.M. Lima, B. Rossi Bergmann, V.L. de Moraes, S.S. Costa, Planta Med. 66 (2000) 134–137.
[CrossRef] U. Supratman, T. Fujita, K. Akiyama, H. Hayashi, A. Murakami, H. Sakai, K. Koshimizu, H. Ohigashi, Biosci. Biotechnol. Biochem. 65 (2001) 947–949.
[CrossRef] U. Supratman, T. Fujita, K. Akiyama, H. Hayashi, Biosci. Biotechnol. Biochem. 64 (2000) 1310–1312.
[CrossRef] K.N. Gaind, R.L. Gupta, Phytochemistry 11 (1972) 1500–1502.
[CrossRef] K.N. Gaind, R.L. Gupta, Planta Med. 23 (1973) 149–153.
[CrossRef] M.F. Muzitano, A.L.W. Tinocoa, C. Guetteb, C.R. Kaiserc, B. Rossi-Bergmann, S.S. Costa, Phytochemistry 67 (2006) 2071–2077.
[CrossRef] V.P.S. Varier, Indian Medicinal Plants. A Compendium of 500 Species, Vol. 3, Orient Longman, Chennai, 2002, p. 282–284.
S. Pal, A.K. Nag Chaudhari, Med. Sci. Res. 17 (1989) 561–562.
U.K. Gilhotra, A.J.M. Christina, Int. J. Pharm. Sci. Rev. Res. 10 (2011) 1870–192.
R. Saleem, M. Ahmed, S.A. Hussain, A.M. Qazi, S.I. Ahmed, M.H. Qazi, M. Ali, S. Faizi, S. Akhtar, S.N. Hussain, Planta Med. 65 (1999) 331–334.
[CrossRef] A. Gupta, R. Gupta, Phytochemistry 46 (1997) 827–831
[CrossRef] S. Faizi, M. Ali, Planta Med. 65 (1999) 383–386.
[CrossRef] V. Verma, S.S. Jalalpure, A. Sahu, L.K. Bhardwaj, P. Yache, Int. Pharm. Sci. 1 (2011) 62–68.
C.A. Lans, J. Ethanobiol. Ethnomed. 2 (2006) 45.
[CrossRef] S. Darshan, R. Doreswamy, Phytother. Res. 18 (2004) 343–357.
[CrossRef] R. Singh, A. Bagachi, A. Semwal, S. Kaur, A. Bharadwaj, J. Medicinal Plants Res. 7 (2013) 461–469.
B. Enkhmaa, J. Nutr. 135 (2005) 729–734.
K. Doi, T. Kojima, M. Makino, Y. Kimura, Y. Fujimoto, Chem. Pharm. Bull. 49 (2001) 151–153.
[CrossRef] B. Devi, N. Sharma, D. Kumar, K. Jeet, Int. J. Pharm. Pharm. Sci. 5 (2013) 14–18.
J. Du, Z.D. He, R.W. Jiang, W.C. Ye, H.X. Xu, P.P.H. But, Phytochemistry 62 (2003) 1235–1238.
[CrossRef] M. Rajani, S.K. Niranjan, Phytochemical Standardization of Herbal Drugs and Polyherbal Formulations, in: K.G. Ramawat, J.M. Merillon (eds.) Bioactive Molecules and Medicinal Plants, Springer-Verlag, Berlin, Heidelberg, 2008, pp. 349–369.
S. Anandjiwala, H. Srinivasa, M. Rajani, Chromatographia 66 (2007) 725–734.
[CrossRef]