An UPLC-MSTOF investigation of the leaves of Arrabidaea patellifera

F Martin ¹, AE Hay ¹, MP Gupta ², K Hostettmann ¹

• ¹Laboratory of Pharmacognosy and Phytochemistry, School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Quai Ernest-Ansermet 30, CH-1211 Geneva 4, Switzerland
• ²Center for Pharmacognostic Research on Panamanian Flora (CIFLORPAN), College of Pharmacy, University of Panama, Panama City, Panama

Abstract

As a part of our ongoing investigations on Panamean Bignoniaceae, several species were submitted to rapid TLC autobiographical tests, against Cladosporium cucumerinum and radical scavenging activity. Arrabidaea patellifera (Schlecht) Sandw, a liana which grows from lowlands to mountains forest, has been selected due to the good activity observed against Cladosporium, and moreover this plant has never been investigated.
The methanol extract has been prepurified by SPE, separated and analyzed by Ultra-Performance Liquid Chromatography coupled with High-Resolution Time of Flight Mass Spectroscopy (UPLC-TOF). Comparing datas (UV, exact mass) with those of Arrabidaea samydoides (1), several compounds with radical scavenging activity have been identified such as mangiferin, muraxanthone and other C-glucosylxanthones.
The dichloromethane extract of A. patelliferas has been fractionated by centrifugal partition chromatography (CPC), medium pressure chromatography (MPLC) and semi-preparative HPLC. Several products have been isolated, such as a flavonoid (chrysin), an antocyanidine and triterpens (like ursolic acid).
Reference: 1. Pauletti, P. et al. (2003), J. Nat. Prod. 66: 1384–1387.