Comparative cytotoxicity of Glycyrrhiza glabra roots from different geographical origins against immortal human keratinocyte (HaCaT), lung adenocarcinoma (A549) and liver carcinoma (HepG2) cells

Phytotherapy Research

Volume 29, Issue 6, 1 June 2015, Pages 944-948

Basar, N.^ab, 

Oridupa, O.A.^ac, 

Ritchie, K.J.^a, 

Nahar, L.^a, 

Osman, N.M.M.^a, 

Stafford, A.^d, 

Kushiev, H.^e, 

Kan, A.^f, 

Sarker, S.D.^a 

 

^a  Medicinal Chemistry and Natural Products Research Group, School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, Byrom Street, Liverpool, United Kingdom
^b  Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, Johor Bahru, Johor, Malaysia
^c  Department of Veterinary Physiology, Biochemistry and Pharmacology, Faculty of Veterinary Medicine, University of Ibadan, Ibadan, Nigeria 

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Abstract

Glycyrrhiza glabra L. (Fabaceae), commonly known as 'liquorice', is a well-known medicinal plant. Roots of this plant have long been used as a sweetening and flavouring agent in food and pharmaceutical products, and also as a traditional remedy for cough, upper and lower respiratory ailments, kidney stones, hepatitis C, skin disorder, cardiovascular diseases, diabetes, gastrointestinal ulcers and stomach ache. Previous pharmacological and clinical studies have revealed its antitussive, antiinflammatory, antiviral, antimicrobial, antioxidant, immunomodulatory, hepatoprotective and cardioprotective properties. While glycyrrhizin, a sweet-tasting triterpene saponin, is the principal bioactive compound, several bioactive flavonoids and isoflavonoids are also present in the roots of this plant. In the present study, the cytotoxicity of the methanol extracts of nine samples of the roots of G.-glabra, collected from various geographical origins, was assessed against immortal human keratinocyte (HaCaT), lung adenocarcinoma (A549) and liver carcinoma (HepG2) cell lines using the in vitro 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazoliumbromide cell toxicity/viability assay. Considerable variations in levels of cytotoxicity were observed among various samples of G.-glabra. Copyright © 2015 John Wiley & Sons, Ltd.

Author keywords

cancer; cytotoxicity; Fabaceae; Glycyrrhiza glabra; liquorice; MTT

Indexed keywords

EMTREE drug terms: Glycyrrhiza glabra extract; flavonoid; plant extract; saponin; triterpene

EMTREE medical terms: A549 cell line; antineoplastic activity; Article; cell viability assay; controlled study; cytotoxicity assay; drug cytotoxicity; geographic origin; Glycyrrhiza glabra; HaCaT cell; HepG2 cell line; human; IC50; in vitro study; keratinocyte; MTT assay; plant root; adenocarcinoma; chemical structure; chemistry; comparative study; drug effects; geography; Glycyrrhiza; liver cell carcinoma; liver tumor; lung tumor; pathology; tumor cell line

Species Index: Fabaceae; Glycyrrhiza glabra; Hepatitis C virus

MeSH: Adenocarcinoma; Carcinoma, Hepatocellular; Cell Line, Tumor; Flavonoids; Geography; Glycyrrhiza; Humans; Inhibitory Concentration 50; Keratinocytes; Liver Neoplasms; Lung Neoplasms; Molecular Structure; Plant Extracts; Plant Roots; Saponins; Triterpenes
Medline is the source for the MeSH terms of this document.

Chemicals and CAS Registry Numbers: saponin, 8047-15-2;Flavonoids; Plant Extracts; Saponins; Triterpenes

ISSN: 0951418X CODEN: PHYRESource Type: Journal Original language: English

DOI: 10.1002/ptr.5329 PubMed ID: 25779384Document Type: Article

Publisher: John Wiley and Sons Ltd

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  Sarker, S.D.; Medicinal Chemistry and Natural Products Research Group, School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, Byrom Street, United Kingdom
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