43
Volume 95, 1 June 2016, Pages 200-207
Psidium guajava L. and Psidium brownianum Mart ex DC.: Chemical composition and anti - Candida effect in association with fluconazole (Article)
a Department of Biological Sciences, Regional University of Cariri, Crato, Ceará, Brazilb Department of Biological Chemistry, Regional University of Cariri, Crato, Ceará, Brazilc Federal University of the Semi Arid, Mossoró, Rio Grande do Norte, Brazil
Abstract
The therapeutic combinations have been increasingly used against fungal resistance. Natural products have been evaluated in combination with pharmaceutical drugs in the search for new components able to work together in order to neutralize the multiple resistance mechanisms found in yeasts from the genus Candida. The aqueous and hydroethanolic extracts from Psidium brownianum Mart ex DC. and Psidium guajava L. species were evaluated for their potential to change the effect of commercial pharmaceutical drugs against Candida albicans and Candida tropicalis strains. The tests were performed according to the broth microdilution method. Plate readings were carried out by spectrophotometry, and the data generated the cell viability curve and IC50 of the extracts against the yeasts. A chemical analysis of all the extracts was performed for detection and characterization of the secondary metabolites. The total phenols were quantified in gallic acid eq/g of extract (GAE/g) and the phenolic composition of the extracts was determined by HPLC. Fluconazole and all extracts presented high Minimum Inhibitories Concentrations (MICs). However, when associated with the extracts at sub-inhibitory concentrations (MIC/16), fluconazole had its effect potentiated. A synergistic effect was observed in the combination of fluconazole with Psidium brownianum extracts against all Candida strains. However, for Psidium guajava extracts the synergistic effect was produced mainly against the Candida albicans LM77 and Candida tropicalis INCQS 400042 strains. The IC50 values of fluconazole ranged from 19.22 to 68.1 μg/mL when it was used alone, but from 2.2 to 45.4 μg/mL in the presence of the extracts. The qualitative chemical characterization demonstrated the presence of phenols, flavonoids and tannins among the secondary metabolites. The concentration of total phenols ranged from 49.25 to 80.77 GAE/g in the P. brownianum extracts and from 68.06 to 82.18 GAE/g in the P. guajava extracts. Our results indicated that both P. brownianum and P. guajava extracts are effective on potentiating the effect of fluconazole, and therefore, these plants have the potential for development of new effective drugs for treating fungal infections. © 2016 Elsevier Ltd.
Author keywords
Antifungal effect modulation; Phytochemical characterization; Psidium species; Yeasts
ISSN: 08824010 CODEN: MIPAE Source Type: Journal Original language: English
DOI: 10.1016/j.micpath.2016.04.013 Document Type: Article
Publisher: Academic Press
3
(2014)
Antimicrobial Resistance: Global Report on Surveillance .
Cited 533 times .
World Health Organization
7
Chen, X., Ren, B., Chen, M., Liu, M.-X., Ren, W., Wang, Q.-X., Zhang, L.-X., (...), Yan, G.-Y.
ASDCD: Antifungal Synergistic Drug Combination Database (2014)
PLoS ONE , 9 (1), art. no. e86499.
Cited 7 times .
http://www.plosone.org/article/fetchObject.action?uri=info%3Adoi%2F10.1371%2Fjournal.pone.0086499&representation=PDF doi: 10.1371/journal.pone.0086499
8
Calixto Júnior, J.T.
Phytochemical analysis and modulation of antibiotic activity by Luehea paniculata Mart. & Zucc. (Malvaceae) in multiresistant clinical isolates of Candida Spp
(2015)
Biomed. Res. Int. , p. 2015.
9
Souza, T.M., Morais-Braga, M.F.B., Costa, J.G.M., Saraiva, A.A.F., Coutinho, H.D.M.
Enhancement of antimicrobial activity of antibiotics and antifungals by the use of natural products from Pityrogramma calomelanos (L.) Link (2012)
Archives of Biological Sciences , 64 (1), pp. 43-48.
Cited 2 times .
http://archonline.bio.bg.ac.rs/VOL64/SVESKA1/06%20-%20Souza.pdf doi: 10.2298/ABS1201043S
10
Spitzer, M., Griffiths, E., Blakely, K.M., Wildenhain, J., Ejim, L., Rossi, L., De Pascale, G., (...), Wright, G.D.
Cross-species discovery of syncretic drug combinations that potentiate the antifungal fluconazole (2011)
Molecular Systems Biology , 7, art. no. 499.
Cited 54 times .
doi: 10.1038/msb.2011.31
11
Matos, F.J.A.
(2002)
Living Pharmacies Fortaleza: Publisher UFC, Português
12
Matos, F.J.A.
(2009)
Introduction to Experimental Phytochemical .
Cited 11 times .
Fortaleza: Publisher UFC, Português
14
Kamdem, J.P., Olalekan, E.O., Hassan, W., Kade, I.J., Yetunde, O., Boligon, A.A., Athayde, M.L., (...), Rocha, J.B.T.
Trichilia catigua (Catuaba) bark extract exerts neuroprotection against oxidative stress induced by different neurotoxic agents in rat hippocampal slices (2013)
Industrial Crops and Products , 50, pp. 625-632.
Cited 14 times .
doi: 10.1016/j.indcrop.2013.07.033
15
Da Silva, A.R.H., Moreira, L.D.R., Brum, E.D.S., De Freitas, M.L., Boligon, A.A., Athayde, M.L., Roman, S.S., (...), Brandão, R.
Biochemical and hematological effects of acute and sub-acute administration to ethyl acetate fraction from the stem bark Scutia buxifolia Reissek in mice (2014)
Journal of Ethnopharmacology , 153 (3), pp. 908-916.
Cited 7 times .
www.elsevier.com/locate/jethpharm doi: 10.1016/j.jep.2014.03.063
17
Morais-Braga, M.F.B.
HPLC-DAD, fungistatic and anti-morphogenical analysis of extracts from Psidium brownianum Mart. Ex DC. against yeasts of the genus Candida
(2015)
Int. J. Food Prop.
18
Javadpour, M.M., Juban, M.M., Lo, W.-C.J., Bishop, S.M., Alberty, J.B., Cowell, S.M., Becker, C.L., (...), McLaughlin, M.L.
De novo antimicrobial peptides with low mammalian cell toxicity (1996)
Journal of Medicinal Chemistry , 39 (16), pp. 3107-3113.
Cited 244 times .
doi: 10.1021/jm9509410
19
Coutinho, H.D.M., Costa, J.G.M., Lima, E.O., Falcão-Silva, V.S., Siqueira Jr., J.P.
Enhancement of the antibiotic activity against a multiresistant Escherichia coli by Mentha arvensis L. and chlorpromazine (2008)
Chemotherapy , 54 (4), pp. 328-330.
Cited 88 times .
doi: 10.1159/000151267
21
Fica, A.
Tratamiento de infecciones fúngicas sistémicas Primera parte: fluconazol, itraconazol y voriconazol
(2004)
Rev. Chil. Infectol. , 21 (1), pp. 26-38.
Cited 8 times .
22
Goel, N., Gagneja, D., Chaudhary, U., Agarwal, R.
Fluconazole Resistance among Candida species Causing vulvovaginitis
(2012)
Res. Rev. J. Microbiol. Virol. , 2 (2).
23
Nodoushan, A.J., Dehghani, M., Mirbagheri, S.M.
In vitro antifungal effect of aqueous Garlic (Allium sativum) extract and its combination with fluconazole against five common clinical Candida isolated from candidiasis lesions
(2007)
J. Kerman Univ. Med. Sci. , 14 (3), pp. 153-162.
24
Guo, N., Wu, X., Yu, L., Liu, J., Meng, R., Jin, J., Lu, H., (...), Deng, X.
In vitro and in vivo interactions between fluconazole and allicin against clinical isolates of fluconazole-resistant Candida albicans determined by alternative methods (2010)
FEMS Immunology and Medical Microbiology , 58 (2), pp. 193-201.
Cited 17 times .
http://www.wiley.com/bw/journal.asp?ref=0928-8244 doi: 10.1111/j.1574-695X.2009.00620.x
25
Saad, A., Fadli, M., Bouaziz, M., Benharref, A., Mezrioui, N.-E., Hassani, L.
Anticandidal activity of the essential oils of thymus maroccanus and thymus broussonetii and their synergism with amphotericin B and fluconazol (2010)
Phytomedicine , 17 (13), pp. 1057-1060.
Cited 29 times .
doi: 10.1016/j.phymed.2010.03.020
26
Dai, L., Zang, C., Tian, S., Liu, W., Tan, S., Cai, Z., Ni, T., (...), Jiang, Y.
Design, synthesis, and evaluation of caffeic acid amides as synergists to sensitize fluconazole-resistant Candida albicans to fluconazole (2015)
Bioorganic and Medicinal Chemistry Letters , 25 (1), pp. 34-37.
http://www.journals.elsevier.com/bioorganic-and-medicinal-chemistry-letters/ doi: 10.1016/j.bmcl.2014.11.022
28
Da Silva, C.R., De Andrade Neto, J.B., De Sousa Campos, R., Figueiredo, N.S., Sampaio, L.S., Magalhães, H.I.F., Cavalcanti, B.C., (...), Nobre Jr., H.V.
Synergistic effect of the flavonoid catechin, quercetin, or epigallocatechin gallate with fluconazole induces apoptosis in Candida tropicalis resistant to fluconazole (2014)
Antimicrobial Agents and Chemotherapy , 58 (3), pp. 1468-1478.
Cited 8 times .
http://aac.asm.org/content/58/3/1468.full.pdf+html doi: 10.1128/AAC.00651-13
29
Alves, C.T., Ferreira, I.C.F.R., Barros, L., Silva, S., Azeredo, J., Henriques, M.
Antifungal activity of phenolic compounds identified in flowers from North Eastern Portugal against Candida species (2014)
Future Microbiology , 9 (2), pp. 139-146.
Cited 4 times .
doi: 10.2217/fmb.13.147
30
Fu, J., Cheng, K., Zhang, Z.-m., Fang, R.-q., Zhu, H.-l.
Synthesis, structure and structure-activity relationship analysis of caffeic acid amides as potential antimicrobials (2010)
European Journal of Medicinal Chemistry , 45 (6), pp. 2638-2643.
Cited 35 times .
doi: 10.1016/j.ejmech.2010.01.066
32
Bisignano, G., Sanogo, R., Marino, A., Aquino, R., D'Angelo, V., Germanò, M.P., De Pasquale, R., (...), Pizza, C.
Antimicrobial activity of Mitracarpus scaber extract and isolated constituents (2000)
Letters in Applied Microbiology , 30 (2), pp. 105-108.
Cited 44 times .
33
Thati, B., Noble, A., Rowan, R., Creaven, B.S., Walsh, M., McCann, M., Egan, D., (...), Kavanagh, K.
Mechanism of action of coumarin and silver(I)-coumarin complexes against the pathogenic yeast Candida albicans (2007)
Toxicology in Vitro , 21 (5), pp. 801-808.
Cited 53 times .
doi: 10.1016/j.tiv.2007.01.022
34
Yun, J., Lee, H., Ko, H.J., Woo, E.-R., Lee, D.G.
Fungicidal effect of isoquercitrin via inducing membrane disturbance (2015)
Biochimica et Biophysica Acta - Biomembranes , 1848 (2), pp. 695-701.
Cited 3 times .
www.elsevier.com/locate/bbamem doi: 10.1016/j.bbamem.2014.11.019
35
Tambe, R., Singhal, R.G., Bhise, K., Kulkarni, M.
Phytochemical screening and HPTLC fingerprinting of leaf extracts of Psidium guajava Linn
(2014)
J. Pharmacogn. Phytochem. , 3 (1), pp. 52-56.
36
Dakappa, S.S., Adhikari, R., Timilsina, S.S., Sajjekhan, S.
A review on the medicinal plant Psidium guajava Linn. (Myrtaceae)
(2013)
J. Drug Deliv. Ther. , 3 (2).
Cited 9 times .
37
Rauha, J.-P., Remes, S., Heinonen, M., Hopia, A., Kähkönen, M., Kujala, T., Pihlaja, K., (...), Vuorela, P.
Antimicrobial effects of Finnish plant extracts containing flavonoids and other phenolic compounds (2000)
International Journal of Food Microbiology , 56 (1), pp. 3-12.
Cited 508 times .
doi: 10.1016/S0168-1605(00)00218-X
38
Gehrke, I.T.S., Neto, A.T., Pedroso, M., Mostardeiro, C.P., Da Cruz, I.B.M., Silva, U.F., Ilha, V., (...), Morel, A.F.
Antimicrobial activity of Schinus lentiscifolius (Anacardiaceae) (2013)
Journal of Ethnopharmacology , 148 (2), pp. 486-491.
Cited 13 times .
doi: 10.1016/j.jep.2013.04.043
39
Özçelik, B., Kartal, M., Orhan, I.
Cytotoxicity, antiviral and antimicrobial activities of alkaloids, flavonoids, and phenolic acids (2011)
Pharmaceutical Biology , 49 (4), pp. 396-402.
Cited 64 times .
doi: 10.3109/13880209.2010.519390
40
Teodoro, G.R., Brighenti, F.L., Delbem, A.C.B., Delbem, Á.C.B., Khouri, S., Gontijo, A.V.L., Pascoal, A.C.R.F., (...), Koga-Ito, C.Y.
Antifungal activity of extracts and isolated compounds from Buchenavia tomentosa on Candida albicans and non-albicans (2015)
Future Microbiology , 10 (6), pp. 917-927.
Cited 2 times .
http://www.futuremedicine.com/loi/fmb doi: 10.2217/fmb.15.20
43
Salazar-Aranda, R., Granados-Guzmán, G., Pérez-Meseguer, J., González, G.M., De Torres, N.W.
Activity of polyphenolic compounds against candida glabrata (2015)
Molecules , 20 (10), pp. 17903-17912.
http://www.mdpi.com/1420-3049/20/10/17903/pdf doi: 10.3390/molecules201017903
Morais-Braga, M.F.B.; Regional University of Cariri, Center for Biological and Health Sciences, Department of Biological Sciences, Laboratory of Microbiology and Molecular Biology, Av. Cel. Antonio Luiz, 1161. Pepper, Brazil; email:
flavianamoraisb@yahoo.com.br © Copyright 2016 Elsevier B.V., All rights reserved.
Microbial Pathogenesis
Volume 95, 1 June 2016, Pages 200-207
Psidium guajava L. and Psidium brownianum Mart ex DC.: Chemical composition and anti - Candida effect in association with fluconazole (Article)
a Department of Biological Sciences, Regional University of Cariri, Crato, Ceará, Brazilb Department of Biological Chemistry, Regional University of Cariri, Crato, Ceará, Brazilc Federal University of the Semi Arid, Mossoró, Rio Grande do Norte, Brazil
Abstract
The therapeutic combinations have been increasingly used against fungal resistance. Natural products have been evaluated in combination with pharmaceutical drugs in the search for new components able to work together in order to neutralize the multiple resistance mechanisms found in yeasts from the genus Candida. The aqueous and hydroethanolic extracts from Psidium brownianum Mart ex DC. and Psidium guajava L. species were evaluated for their potential to change the effect of commercial pharmaceutical drugs against Candida albicans and Candida tropicalis strains. The tests were performed according to the broth microdilution method. Plate readings were carried out by spectrophotometry, and the data generated the cell viability curve and IC50 of the extracts against the yeasts. A chemical analysis of all the extracts was performed for detection and characterization of the secondary metabolites. The total phenols were quantified in gallic acid eq/g of extract (GAE/g) and the phenolic composition of the extracts was determined by HPLC. Fluconazole and all extracts presented high Minimum Inhibitories Concentrations (MICs). However, when associated with the extracts at sub-inhibitory concentrations (MIC/16), fluconazole had its effect potentiated. A synergistic effect was observed in the combination of fluconazole with Psidium brownianum extracts against all Candida strains. However, for Psidium guajava extracts the synergistic effect was produced mainly against the Candida albicans LM77 and Candida tropicalis INCQS 400042 strains. The IC50 values of fluconazole ranged from 19.22 to 68.1 μg/mL when it was used alone, but from 2.2 to 45.4 μg/mL in the presence of the extracts. The qualitative chemical characterization demonstrated the presence of phenols, flavonoids and tannins among the secondary metabolites. The concentration of total phenols ranged from 49.25 to 80.77 GAE/g in the P. brownianum extracts and from 68.06 to 82.18 GAE/g in the P. guajava extracts. Our results indicated that both P. brownianum and P. guajava extracts are effective on potentiating the effect of fluconazole, and therefore, these plants have the potential for development of new effective drugs for treating fungal infections. © 2016 Elsevier Ltd.
Author keywords
Antifungal effect modulation; Phytochemical characterization; Psidium species; Yeasts
ISSN: 08824010 CODEN: MIPAE Source Type: Journal Original language: English
DOI: 10.1016/j.micpath.2016.04.013 Document Type: Article
Publisher: Academic Press
Morais-Braga, M.F.B.; Regional University of Cariri, Center for Biological and Health Sciences, Department of Biological Sciences, Laboratory of Microbiology and Molecular Biology, Av. Cel. Antonio Luiz, 1161. Pepper, Brazil; email:
flavianamoraisb@yahoo.com.br © Copyright 2016 Elsevier B.V., All rights reserved.
Microbial Pathogenesis
Volume 95, 1 June 2016, Pages 200-207
Psidium guajava L. and Psidium brownianum Mart ex DC.: Chemical composition and anti - Candida effect in association with fluconazole (Article)
a Department of Biological Sciences, Regional University of Cariri, Crato, Ceará, Brazilb Department of Biological Chemistry, Regional University of Cariri, Crato, Ceará, Brazilc Federal University of the Semi Arid, Mossoró, Rio Grande do Norte, Brazil
Abstract
The therapeutic combinations have been increasingly used against fungal resistance. Natural products have been evaluated in combination with pharmaceutical drugs in the search for new components able to work together in order to neutralize the multiple resistance mechanisms found in yeasts from the genus Candida. The aqueous and hydroethanolic extracts from Psidium brownianum Mart ex DC. and Psidium guajava L. species were evaluated for their potential to change the effect of commercial pharmaceutical drugs against Candida albicans and Candida tropicalis strains. The tests were performed according to the broth microdilution method. Plate readings were carried out by spectrophotometry, and the data generated the cell viability curve and IC50 of the extracts against the yeasts. A chemical analysis of all the extracts was performed for detection and characterization of the secondary metabolites. The total phenols were quantified in gallic acid eq/g of extract (GAE/g) and the phenolic composition of the extracts was determined by HPLC. Fluconazole and all extracts presented high Minimum Inhibitories Concentrations (MICs). However, when associated with the extracts at sub-inhibitory concentrations (MIC/16), fluconazole had its effect potentiated. A synergistic effect was observed in the combination of fluconazole with Psidium brownianum extracts against all Candida strains. However, for Psidium guajava extracts the synergistic effect was produced mainly against the Candida albicans LM77 and Candida tropicalis INCQS 400042 strains. The IC50 values of fluconazole ranged from 19.22 to 68.1 μg/mL when it was used alone, but from 2.2 to 45.4 μg/mL in the presence of the extracts. The qualitative chemical characterization demonstrated the presence of phenols, flavonoids and tannins among the secondary metabolites. The concentration of total phenols ranged from 49.25 to 80.77 GAE/g in the P. brownianum extracts and from 68.06 to 82.18 GAE/g in the P. guajava extracts. Our results indicated that both P. brownianum and P. guajava extracts are effective on potentiating the effect of fluconazole, and therefore, these plants have the potential for development of new effective drugs for treating fungal infections. © 2016 Elsevier Ltd.
Author keywords
Antifungal effect modulation; Phytochemical characterization; Psidium species; Yeasts
ISSN: 08824010 CODEN: MIPAE Source Type: Journal Original language: English
DOI: 10.1016/j.micpath.2016.04.013 Document Type: Article
Publisher: Academic Press
Morais-Braga, M.F.B.; Regional University of Cariri, Center for Biological and Health Sciences, Department of Biological Sciences, Laboratory of Microbiology and Molecular Biology, Av. Cel. Antonio Luiz, 1161. Pepper, Brazil; email:
flavianamoraisb@yahoo.com.br © Copyright 2016 Elsevier B.V., All rights reserved.
