Associations between urinary organophosphate pesticide metabolite levels and reproductive parameters in men from an infertility clinic

Environmental Research

Volume 137, February 01, 2015, Pages 292-298

  (Article)

Melgarejo, M.^ab, 

Mendiola, J.^a , 

Koch, H.M.^c, 

Moñino-García, M.^a, 

Noguera-Velasco, J.A.^b, 

Torres-Cantero, A.M.^ade

 

^a  Division of Preventive Medicine and Public Health, Department of Health and Social Sciences, University of Murcia School of Medicine, Espinardo, Murcia, Spain
^b  Department of Laboratory Medicine, Virgen de la Arrixaca University Hospital, Palmar, Murcia, Spain
^c  Inst. for Prevention and Occupational Medicine of the German Social Accident Insurance, Inst. of the Ruhr-Universität Bochum (IPA), Bochum, Germany 

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Abstract

Organophosphate (OP) pesticides are compounds used for pest control at home or in agriculture activities. Almost all OP pesticides are metabolized to at least one of six possible dialkylphosphates (DAPs). Despite wide use, their potential effects on human reproductive health have not yet been fully characterized. The aim of this study was to evaluate the associations between urinary concentrations of six DAP metabolites and reproductive parameters in men. All men were attended aninfertility clinic and provided urine, serum and semen samples on the same day. Six DAP metabolites were measured in urine (dimethylphosphate [DMP], dimethylthiophosphate [DMTP], dimethyldithiophosphate [DMDTP], diethylphosphate [DEP], diethylthiophosphate [DETP], and diethyldithiophosphate [DEDTP]). Sperm quality was assessed by measuring volume, concentration, total sperm count (TSC), motility and morphology, and serum samples were analyzed for reproductivehormones, including follicle-stimulating hormone (FSH), luteinizing hormone (LH), testosterone, prolactin and estradiol. Pearson correlations were used for unadjusted analyses, and multiple linear regression analysis was performed controlling for appropriate covariates. All men presented detectable concentrations of at least one urinary OP metabolite. After adjustment by important covariates, there was a significant positive association between DEDTP concentrations and LH [(β)=11.4; 95% CI 0.81-22.1] as well as FSH levels [(β)=3.2; 95% CI 0.08-6.2]. Sperm concentration and TSC were both significantly inversely associated with DMP, DMDP, DMDTP and ∑DAP in multivariate analysis. Besides, there was a significant inverse association between percentage of motile sperm and DMTP, DMDTP and DEP metabolite concentrations. Our results suggest that exposure to OP pesticides may be associated with decreased sperm counts and motility and altered reproductivehormone levels in male partners of couples seeking for infertility treatment. However, further studies are warranted to confirm and extent these findings. © 2015 Elsevier Inc.

Author keywords

Endocrine disruptors; Organophosphate pesticides; Reproductive hormones; Semen quality

Indexed keywords

EMTREE drug terms: diethyldithiophosphate; dimethyldithiophosphate; dimethylphosphate; dimethylthiophosphate; estradiol; follitropin; luteinizing hormone; organophosphate pesticide; prolactin; testosterone; unclassified drug; gonadotropin; organophosphate; pesticide; pollutant; sex hormone

GEOBASE Subject Index: concentration (composition); endocrine disruptor; hormone; metabolite; motility; organophosphate; parameterization; pest control; pesticide; urine

EMTREE medical terms: adult; Article; cell structure; human; human cell; male; male infertility; priority journal; sperm morphology; spermatozoon count; spermatozoon motility; blood; cross-sectional study; environmental exposure; environmental monitoring; mass fragmentography; pollutant; semen analysis; Spain; urine

MeSH: Adult; Cross-Sectional Studies; Environmental Exposure; Environmental Monitoring; Environmental Pollutants; Gas Chromatography-Mass Spectrometry; Gonadal Steroid Hormones; Gonadotropins, Pituitary; Humans; Male; Organophosphates; Pesticides; Semen Analysis; Spain; Sperm Count
Medline is the source for the MeSH terms of this document.

Chemicals and CAS Registry Numbers: estradiol, 50-28-2; follitropin, 9002-68-0; luteinizing hormone, 39341-83-8, 9002-67-9; prolactin, 12585-34-1, 50647-00-2, 9002-62-4; testosterone, 58-22-0; gonadotropin, 63231-54-9;Environmental Pollutants; Gonadal Steroid Hormones; Gonadotropins, Pituitary; Organophosphates; Pesticides

ISSN: 00139351 CODEN: ENVRASource Type: Journal Original language: English

DOI: 10.1016/j.envres.2015.01.004 PubMed ID: 25601731Document Type: Article

Publisher: Academic Press Inc.

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  Mendiola, J.; Division of Preventive Medicine and Public Health, Department of Health and Social Sciences, University of Murcia School of Medicine, Spain
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