Volume 210, 1 November 2016, Pages 558–565
A. Mataa,
- a Faculdade de Farmácia da Universidade de Lisboa, Av. Prof. Gama Pinto, 1649-019 Lisboa, Portugal
- b Instituto de Biologia Experimental e Tecnológica, Apartado 12, 2780-901 Oeiras, Portugal
- c Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Av. da República, 2780-157 Oeiras, Portugal
Highlights
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- Composition of Opuntia ficus-indica juice was characterized by LC–DAD–ESI-MS/MS.
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- Phenolic and phenylpyruvic acids and derivatives were detected.
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- Isorhamnetin derivatives were the dominant flavonol glycosides identified.
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- Six betaxanthins and three betacyanin were detected in the samples.
- •
- Derivative compounds from piscidic acid were tentatively characterized.
Abstract
Opuntia
spp. fruits are considered as health promoting foods due to the
diversity of bioactive molecules found in these fruits. The composition
in organic acids, flavonols and betalains in the Opuntia ficus-indica
juice from a region of Portugal was accomplished for the first time by
liquid chromatography and tandem mass spectrometry using an electrospray
ionization source operating in negative and positive mode. The
methodology used allowed the detection of 44 compounds, from which 32
were identified. Isorhamnetin derivatives were the dominant flavonol
glycosides. A total of 9 betalains including 6 betaxanthins and 3
betacyanin were also detected in the fruit juice samples and
indicaxanthin, betanin and isobetanin were the major pigments. Phenolic
acid and phenylpyruvic acid derivatives were also identified. To our
knowledge, it is the first time derivative compounds from piscidic acid,
phenolic compounds and betalains are characterized in cactus pear juice
using a single LC–DAD–ESI-MS/MS method.
Keywords
- Opuntia spp.;
- LC–DAD–ESI-MS/MS;
- Phenols;
- Phenylpyruvic acid derivatives;
- Organic acids;
- Betalains
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