- 1Process
Design and Development Department, Egyptian Petroleum Research
Institute (EPRI), Nasr City, Cairo, Egypt. Electronic address:
raiediana@yahoo.com.
- 2Terrestrial Biofilms Group, Institute of Microbiology, Friedrich Schiller University Jena (FSU), Jena, Germany.
- 3Nanobiophotonic Department, Leibniz Institute of Photonic Technology Jena (IPHT), Jena, Germany.
- 4Process Design and Development Department, Egyptian Petroleum Research Institute (EPRI), Nasr City, Cairo, Egypt.
- 5National Institute of Laser Enhanced Sciences (NILES), Cairo University, Giza, Egypt.
- 6Terrestrial
Biofilms Group, Institute of Microbiology, Friedrich Schiller
University Jena (FSU), Jena, Germany. Electronic address:
akos-tibor.kovacs@uni-jena.de.
Abstract
The
aim of this study was directed to reveal the repulsive effect of coated
glass slides by quercetin and its bio-inspired titanium oxide and
tungsten oxide nanoparticles on physical surface attachment of Bacillus
subtilis as an ab-initio step of biofilm formation. Nanoparticles were
successfully synthesized using sol-gel and acid precipitation methods
for titanium oxide and tungsten oxide, respectively (in the absence or
presence of quercetin). The anti-adhesive impact of the coated-slides
was tested through the physical attachment of B. subtilis after 24h
using Confocal Laser Scanning Microscopy (CLSM). Here, quercetin was
presented as a bio-route for the synthesis of tungsten mixed oxides
nano-plates at room temperature. In addition, quercetin had an impact on
zeta potential and adsorption capacity of both bio-inspired amorphous
titanium oxide and tungsten oxide nano-plates. Interestingly, our
experiments indicated a contrary effect of quercetin as an anti-adhesive
agent than previously reported. However, its bio-inspired metal oxide
proved their repulsive efficiency. In addition, quercetin-mediated
nano-tungsten and quercetin-mediated amorphous titanium showed
anti-adhesive activity against B. subtilis biofilm.
Copyright © 2016 Elsevier B.V. All rights reserved.
KEYWORDS:
Anti-adhesion; Bacillus subtilis; Biofilm; Metal oxide; Nanoparticles; Quercetin