Environ Toxicol. 2016 Nov;31(11):1329-1336. doi: 10.1002/tox.22138. Epub 2015 Apr 8.
- 1Protein
and Proteomics Research Center for Commercial and Industrial Purposes
(ProCCI), Department of Biochemistry, Faculty of Science, Khon Kaen
University, Khon Kaen, 40002, Thailand.
- 2Department of Clinical Chemistry, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, 40002, Thailand.
- 3Department of Biological Science and Technology, College of Life Science, China Medical University, Taichung, 404, Taiwan.
- 4Sriracha Moda Co., Ltd., Sriracha, Chonburi, 20110, Thailand.
- 5Protein
and Proteomics Research Center for Commercial and Industrial Purposes
(ProCCI), Department of Biochemistry, Faculty of Science, Khon Kaen
University, Khon Kaen, 40002, Thailand. sakdad@kku.ac.th.
Abstract
Matrix metalloproteinases (MMPs) play a key role in cancer
progression, including cell invasion, metastasis, cell growth,
apoptosis, angiogenesis, and cell adhesion. Thus, suppression of the
MMPs activities is crucial for inhibiting cancer cells metastasis. Herein, bioactive agents from crocodile
(Crocodylus siamensis) leukocyte extracts (WBCex) showed the anticancer
activity with HeLa cells and inhibited the migration and invasion
process by reducing gelatinases (MMP-2, MMP-9) activity and their
protein levels. This mechanism is regulated via interfering Ras and p38
signal transduction. Moreover, disrupting VEGF and integrin-signaling
cascade by bioactive agents are the predictable mechanisms that cause
the decreasing of MMP-2 and MMP-9 activity. Hence, bioactive substances
in WBCex may play the mode of action similar with MMPs inhibitor due to
HeLa cell metastasis being suppressed in vitro. © 2015 Wiley
Periodicals, Inc. Environ Toxicol 31: 1329-1336, 2016.
© 2015 Wiley Periodicals, Inc.
KEYWORDS:
MMPs; anticancer peptide; antioxidant peptides; bioactive compounds; leukocyte
