Nat Prod Res. 2015 Jul 29:1-9. [Epub ahead of print]
Kaškonienė V1, Maruška A, Akuņeca I, Stankevičius M, Ragažinskienė O, Bartkuvienė V, Kornyšova O, Briedis V, Ugenskienė R.
Abstract
Since biological activity of medicinal plants
is dependent on cultivation area, climatic conditions, developmental
stage, genetic modifications and other factors, it is important to study
flora present in different growing sites and geographical zones. This
study was focused on screening of antioxidant activity of C.
angustifolium harvested in six different locations in Lithuania.
The total contents of phenolic compounds, flavonoids and
2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity were
evaluated by spectrophotometric methods. A correlation between radical
scavenging activity and total phenolic compounds content was observed
(correlation coefficient 0.98). HPLC with online post-column DPPH
radical scavenging reaction detection was used for the separation of
extracts. Oenothein B, rutin and one unidentified compound were
predominant. Volatile compounds were analysed using solid-phase
microextraction coupled with gas chromatography-mass spectrometry. Based
on the analysis of volatiles, all samples were classified into two
chemotypes: (I) with predominant α- and β-caryophyllenes and (II) with
predominant anethole.
