Saturday, 29 June 2019

16-Hydroxy-Lycopersene, a Polyisoprenoid Alcohol Isolated from Tournefortia hirsutissima, Inhibits Nitric Oxide Production in RAW 264.7 Cells and Induces Apoptosis in Hep3B Cells

Open AccessArticle

Centro de Investigaciones Químicas-IICBA, Universidad Autónoma del Estado de Morelos, Cuernavaca 62209, Morelos, Mexico
Facultad de Farmacia, Universidad Autónoma del Estado de Morelos, Cuernavaca 62209, Morelos, Mexico
Centro de Investigación Biomédica del Sur, Instituto Mexicano del Seguro Social, Xochitepec 62790, Morelos, Mexico
Author to whom correspondence should be addressed.
Academic Editors: Ewa Swiezewska and Liliana Surmacz
Molecules 201924(13), 2366;
Received: 13 June 2019 / Revised: 24 June 2019 / Accepted: 24 June 2019 / Published: 26 June 2019
(This article belongs to the Special Issue Plant Isoprenoids)
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Three polyisoprenoid alcohols were isolated from the leaves of Tournefortia hirsutissima by a bioassay-guided phytochemical investigation. The compounds were identified as 16-hydroxy-lycopersene (Compound 1), (Z8,E3,ω)-dodecaprenol (Compound 2) and (Z9,E3,ω)-tridecaprenol (Compound 3). Compound 1, an unusual polyisoprenoid, was characterized by 1D and 2D NMR. We also determined the absolute configuration at C-16 by the modified Mosher’s method. The in vitro antiproliferative and anti-inflammatory activities of the isolated compounds were evaluated. Among isolates, Compound 1moderately inhibited the nitric oxide production in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. On the other hand, Compound 1 displayed selective antiproliferative activity against HeLa, PC3, HepG2 and Hep3B cancer cells and was less potent against IHH non-cancerous cells. Compound 1 in Hep3B cells showed significant inhibition of cell cycle progression increasing the sub-G1 phase, suggesting cell death. Acridine orange/ethidium bromide staining and Annexin V-FITC/PI staining demonstrated that cell death induced by Compound 1 in cells Hep3B was by apoptosis. Further study showed that apoptosis induced by Compound 1 in Hep3b cells is associated with the increase of the ratio of Bax/Bcl-2, and caspase 3/7 activation. These results suggest that Compound 1 induce apoptotic cell death by the mitochondrial pathway. To our knowledge, this is the first report about the presence of polyprenol Compounds 13 in T. hirsutissima, and the apoptotic and anti-inflammatory action of Compound 1View Full-Text
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Hurtado-Díaz, I.; Sánchez-Carranza, J.N.; Romero-Estrada, A.; González-Maya, L.; González-Christen, J.; Herrera-Ruiz, M.; Alvarez, L. 16-Hydroxy-Lycopersene, a Polyisoprenoid Alcohol Isolated from Tournefortia hirsutissima, Inhibits Nitric Oxide Production in RAW 264.7 Cells and Induces Apoptosis in Hep3B Cells. Molecules 201924, 2366.
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Friday, 28 June 2019

The toxicological assessment of ethanolic whole-plant extract of Eleucine indica in Wistar albino rats

Abstract and figures
Introduction: Eleucine indica is a medicinal plant used by the Ibibios of Nigeria in the treatment of malaria but its safety with chronic use has not been determined. This study was to evaluate the toxicological effects of the extract in adult albino Wistar rats. Methods: The rats of both sexes were randomized into 5 groups of 6 animals per group and orally administered with extract (200, 400 and 600 mg/kg) for groups 2-4, respectively. Group 1 received distilled water (10 mL/kg) orally and served as negative control while group 5 was administered with 100 mg/kg of silymarin orally. Drugs were administered on alternate days for 28 days at 09.00 am. Toxic manifestations and mortality were monitored daily and weight changes of animals were recorded every week. On day 29, after an overnight fast, the animals were weighed, anaesthetized with light chloroform. An autopsy was performed during which any macroscopic abnormalities were noted. The brain, heart, liver, spleen, kidney and lungs were weighed immediately after removal. Samples of these organs were fixed in 10% formalin and kept in that solution for further histopathological examination. Data were analysed using one-way analysis of variance (ANOVA) followed by Tukey Kramer multiple comparison post-test. Results: The results showed that organ weights were not affected but animal weights increased significantly (P < 0.01-0.001). Relative organ weights were not affected. The extract caused, at low doses, slight inflammation of the liver, spleen, lungs, kidneys and brain. With high dose of the extract, the spleen and lungs showed moderate inflammation. The lungs also showed moderate interstitial fibrosis. Conclusion: Based on these results, the plant has a potential to damage the lungs when used on the long term. Its use as herbal remedy should be for short periods at a time.

Tuesday, 25 June 2019

Congratulations - Your article has been cited.

Your work has been cited
Dear Cheryl Lans,
Congratulations - Your article has been cited.
The information is based on your article's DOI (digital object identifier) and CrossRef (
Cited by:
Russo, Rosario et al.:
Natural Product Communications. Volume: 4. Issue: 12. 2009
Katerere, David et al.:
Ethnoveterinary Botanical Medicine. 2010
Neves, Arminda et al.:
The Veterinary Nurse. Volume: 1. Issue: 1. 2010
Umpiérrez, María Laura et al.:
Phytochemistry Reviews. Volume: 10. Issue: 2. 2011
Lans, Cheryl et al.:
Journal of Ethnobiology and Ethnomedicine. Volume: 7. Issue: 1. 2011
Riaz, Muhammad et al.:
Revista Brasileira de Farmacognosia. Volume: 23. Issue: 6. 2013
Kamiie, Yuki et al.:
Transactions of the Materials Research Society of Japan. Volume: 39. Issue: 4. 2014
Godara, R. et al.:
Experimental and Applied Acarology. Volume: 67. Issue: 1. 2015
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Friday, 21 June 2019

CFP: 4th Annual Meeting of the Canadian Society for Environmental Philosophy

4th Annual Meeting of the Canadian Society for Environmental Philosophy/ Société Canadienne de Philosophie Environnementale (in conjunction with the 56th annual meeting of the Western Canadian Philosophical Association) October 25th-27, University of Lethbridge, Lethbridge, Alberta









Submissions of papers from all areas of environmental philosophy are welcome. Papers should be no more than 4,000 words (excluding notes), presentable in 25-30 minutes to allow for commentary and discussion, and prepared for anonymous review. The submission deadline is extended to JULY 15 2019.
Please submit papers in electronic form (PDF) and a brief abstract (no more than 150 words) via EasyChair:
Proposals for panels or symposia are also welcome. Please submit the proposed title of the symposium as well as a collection of abstracts in electronic form (PDF), and a brief abstract (no more than 150 words) indicating that you are proposing a panel/symposium via EasyChair (in the same way as you would submit a paper). Symposia are allotted 2 hours.
Colloquium papers and panels/symposia on environmental topics may be submitted for presentation either on the WCPA main program or the program of the CSEP/SCPE, but will automatically be considered for presentation on both programs. Please indicate if you are submitting with the CSEP/SPCE in mind at the top of your abstract.The CSEP/CSPE will be awarding a Student Essay Prize, so if you are eligible, please indicate this.
For more information about the conference site at the University of Lethbridge, visit:

Saturday, 15 June 2019

Roselle Seed Oil and its Nano-Formulation Alleviated Oxidative Stress, Activated Nrf2 and Downregulated m-RNA Expression Genes of Pro-inflammatory Cytokines in Paracetamol-intoxicated Rat Model

Abstract and figures
Roselle (Hibiscus sabdariffa L.) seeds, traditionally used in liver disorders, have recently attracted more attention as a new source of healthy edible oil with anti-inflammatory and antioxidant activities. However, its hepatoprotective effect has not been explored yet. In the current study, the hepatoprotective potential of roselle seed oil (RSO; 0.6, 4 and 8 mL/kg) and its nano-formulation (RSO-NE; 4 and 8 mL/kg), and their possible underlying mechanism were investigated in a paracetamol-induced hepatotoxicity rat model, compared to silymarin. RSO and RSO-NE protected the liver against paracetamol-intoxication and maintained the overall architecture of liver tissues in a dose dependent manner. Additionally, hepatic nuclear factor-erythroid 2-related factor2 (Nrf2) and glutathione (GSH) increased significantly in pre-treated groups, while malondialdehyde (MDA) decreased. Moreover, RSO and RSO-NE significantly inhibited paracetamol-induced mRNA expression of pro-inflammatory cytokines (TNF-α and IL-6). Chemical analysis of RSO showed fatty acids (mainly linoleic, oleic, palmitic and stearic acids), n-eicosane, β-sitosterol and tocopherols as the major constituents, which contributed synergistically to its protective effect. The efficacy of RSO-NE (8 mL/kg) was superior to its corresponding unformulated oil (0.6 mL/kg), indicating its enhanced bioavailability. These findings encourage the use of RSO in development of health promoting products such as food supplements, functional food and nutraceuticals for the prevention of liver disease.