Polyphenol supplementation alters intramuscular apoptotic signaling following acute resistance exercise

Physiol Rep. 2018 Jan; 6(2): e13552. Published online 2018 Jan 22. doi: 10.14814/phy2.13552 PMCID: PMC5789717 Jeremy R. Townsend,corresponding author 1 Jeffrey R. Stout, 2 Adam R. Jajtner, 3 David D. Church, 2 Kyle S. Beyer, 2 Joshua J. Riffe, 2 Tyler W. D. Muddle, 2 Kelli L. Herrlinger, 4 David H. Fukuda, 2 and Jay R. Hoffman 2 1 Exercise and Nutrition Science Graduate Program, Lipscomb University, Nashville, Tennessee, 2 Institute of Exercise Physiology and Wellness, University of Central Florida, Orlando, Florida, 3 Human Performance Laboratory, Kent State University, Kent, Ohio, 4 Kemin Foods, L.C., Des Moines, Iowa, Jeremy R. Townsend, Email: ude.bmocspil@dnesnwotrj. corresponding authorCorresponding author. *Correspondence Jeremy R. Townsend, Exercise and Nutrition Science Graduate Program, Lipscomb University, 1 University Park Dr, Nashville, TN 37215. Tel: 615‐966‐5618 Fax: 615‐966‐2150 E‐mail: ude.bmocspil@dnesnwotrj, Abstract The purpose of this study was to examine the effects of 28‐days of supplementation with an aqueous proprietary polyphenol blend (PPB) sourced from Camellia sinensis on intramuscular apoptotic signaling following an acute lower‐body resistance exercise protocol and subsequent recovery. Untrained males (n = 38, 21.8 ± 2.7 years, 173.4 ± 7.9 cm, 77.6 ± 14.6 kg) were randomized to PPB (n = 14), placebo (PL; n = 14) or control (CON; n = 10). Participants completed a lower‐body resistance exercise protocol comprised of the squat, leg press, and leg extension exercises. Skeletal muscle microbiopsies were obtained from the vastus lateralis preexercise (PRE), 1‐h (1HR), 5‐h (5HR), and 48‐h (48HR) post‐resistance exercise. Apoptotic signaling pathways were quantified using multiplex signaling assay kits to quantify total proteins (Caspase 3, 8, 9) and markers of phosphorylation status (JNK, FADD, p53, BAD, Bcl‐2). Changes in markers of muscle damage and intramuscular signaling were analyzed via separate repeated measures analysis of variance (ANOVA). Change in Bcl‐2 phosphorylation at 1H was significantly greater in PL compared to CON (P = 0.001). BAD phosphorylation was significantly elevated at 5H in PL compared to PPB (P = 0.015) and CON (P = 0.006). The change in JNK phosphorylation was significantly greater in PPB (P = 0.009), and PL (P = 0.017) compared to CON at 1H, while the change for PL was elevated compared to CON at 5H (P = 0.002). A main effect was observed (P < 0.05) at 1H, 5H, and 48H for p53 and Caspase 8, with Caspase 3 and Caspase 9 elevated at 48H. These data indicate that chronic supplementation with PPB alters apoptotic signaling in skeletal muscle following acute muscle‐damaging resistance exercise. Keywords: Black tea extract, EGCG, green tea extract, muscle damage, theaflavins https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5789717/