Abstract
Multiple
drug resistance and increased side effects due to allopathic drugs has
warned scientific community with a global alarm to identify molecules
from natural sources to combat diseases with minimum or no side effects.
The present investigation was aimed to identify and isolate secondary
metabolites from traditionally used Nerium indicum using conventional
column chromatography which led to the isolation of two compounds, C-I
(fractions NB4f1) and C-II (fractions NC13b1). Further characterized, it
is elucidated using spectral data and identified as
N-(4-hydroxy-phenyl)-2-methoxy-2-phenyl-acetamide, molecular formula
C15H15NO3, and molecular weight 257.3 (C-I) and
N-(4-hydroxy-phenyl)-2-phenyl-N-phenylacetyl-acetamide, molecular
formula C22H19NO3, and molecular weight 345.4 (C-II). Further, the
isolated compounds were investigated using in silico approach by
Autodock tool with four different proteins specific for cancer and in
vitro assessed cell proliferation, and apoptosis against human breast
cancer MCF 7 cell line. The results of the in silico model demonstrated
potent binding affinity of both compounds with the proteins representing
that the isolated molecules could be a drug of choice for cancer.
Further, the isolated compounds revealed significant inhibition of cell
proliferation (IC50 values 21 μg/mL for C-I, 19 μg/mL for C-II) with
induced apoptosis with nuclear condensation effect on the MCF 7 cells in
in vitro condition even at very low concentration. Compound treatment
to MCF-7 cell line represented bright fetches indicating condensed
chromatins and higher level of nuclear fragmentation with DAPI staining,
indicating higher cell death due to induced apoptosis and confirmed
using flow cytometry analysis representing inhibition of cell
proliferation at S phase.
https://www.researchgate.net/publication/338281545_Radical_scavenging_and_antiproliferative_effect_of_novel_phenolic_derivatives_isolated_from_Nerium_indicum_against_human_breast_cancer_cell_line_MCF-7-an_in_silico_and_in_vitro_approach
