- 1Department
of Pharmacognosy, Ludwik Rydygier Collegium Medicum, Nicolaus
Copernicus University, 9 Marie Curie-Skłodowska Street, 85-094
Bydgoszcz, Poland.
- 2Department of Pharmaceutical Botany, Medical University of Lublin, 1 Chodźki Street, 20-093 Lublin, Poland.
- 3Department of Pharmacognosy, Collegium Medicum, Jagiellonian University, 9 Medyczna Street, 30-688 Cracow, Poland.
- 4Natural Products Laboratory, Institute of Biology, Leiden University, 2300 RA Leiden, Netherlands.
- 5Department of Biology and Genetics, Medical University of Lublin, 4a Chodźki Street, 20-093 Lublin, Poland.
Abstract
In the past two decades public interest in herbal products has increased significantly in Europe, especially in the plant-based products from non-European traditions. Eleutherococcus senticosus has been used for the treatment of inflammatory diseases, anemia, and rheumatoid arthritis. The Eleutherococcus senticosus
fruits intractum was examined for the content of phenolic acids
(LC-ESI-MS/MS), minerals (AAS), TPC, and TFC (spectrophotometric assay).
The antioxidant activity was determined using free radical scavenging
assay and TLC-DB-DPPH∗ dot-blot test. An anti-Hyal activity
was evaluated by the spectrophotometric assay method. Cytotoxicity
towards HL-60, HL-60/MX1, HL-60/MX2, CEM/C1, and CCRF/CEM leukemic cell
lines was done using trypan blue test. Among eight phenolic acids, trans-caffeic
acid was found in the largest amount (41.2 mg/g DE). The intractum
presented a high amount of macroelements (Ca, Mg, K; 1750, 1300, and
21000 mg/kg) and microelements (Fe, Mn; 32.7, 54.3 mg/kg), respectively.
The content of TPC and TFC was 130 and 92 mg/g DE, respectively. The
intractum showed anti-Hyal activity (2.16-60%) and an antioxidant
capacity (EC50; 52 μg/mL). The intractum most strongly
inhibited the growth of HL-60, HL-60/MX1, and CCRF/CEM. A better
understanding of the intractum health benefits is important in order to
increase its utility and enrich dietary sources of health promoting
compounds.
