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Tuesday 7 February 2017

Prebiotic potential of neutral oligo- and polysaccharides from seed mucilage of Hyptis suaveolens.

2017 Apr 15;221:508-514. doi: 10.1016/j.foodchem.2016.10.075. Epub 2016 Oct 18.


Mueller M1, Čavarkapa A2, Unger FM2, Viernstein H2, Praznik W2.

Author information

  • 1Department of Pharmaceutical Technology and Biopharmaceutics, University of Vienna, Althanstrasse 14, A-1090 Vienna, Austria. Electronic address: monika.mueller@univie.ac.at.
  • 2Department of Pharmaceutical Technology and Biopharmaceutics, University of Vienna, Althanstrasse 14, A-1090 Vienna, Austria.

Abstract

Prebiotics are selectively fermented by the gastrointestinal microflora, resulting in benefits to human health. The seed mucilage of Hyptis suaveolens contains neutral and acidic polysaccharides in a ratio of 1:1. The neutral polysaccharides consist of galactose, glucose and mannose whereas the acidic polysaccharides contain fucose, xylose and 4-O-methylglucuronic acid -residues. The growth of probiotics in the presence of total, acidic or neutral polysaccharides and oligosaccharides was tested using turbidity measurements. The majority (11 out of 14) of the tested probiotic strains significantly grew in the neutral fraction. Growth occurred with some time delay, but may be longer lasting than with other lower molecular prebiotics. The extent of growth increased with neutral polysaccharides from H. suaveolens corresponding to the externally available galactose units (20%). In conclusion, neutral poly- and oligosaccharides from H. suaveolens have a prebiotic potential characterized by a delayed but long lasting effect.
Copyright © 2016 Elsevier Ltd. All rights reserved.

KEYWORDS:

Hyptis suaveolens; Mucilaginous seeds; Oligosaccharides; Polysaccharides; Prebiotics
PMID:
27979234
DOI:
10.1016/j.foodchem.2016.10.075

f 2017 Apr 15;221:1997-2004. doi: 10.1016/j.foodchem.2016.11.087. Epub 2016 Nov 20.

Molecular dimensions and structural features of neutral polysaccharides from the seed mucilage of Hyptis suaveolens L.

Praznik W1, Čavarkapa A2, Unger FM2, Loeppert R2, Holzer W3, Viernstein H2, Mueller M2.

Author information

  • 1Department of Pharmaceutical Technology and Biopharmaceutics, University of Vienna, Althanstrasse 14, A-1090 Vienna, Austria. Electronic address: werner.praznik@univie.ac.at.
  • 2Department of Pharmaceutical Technology and Biopharmaceutics, University of Vienna, Althanstrasse 14, A-1090 Vienna, Austria.
  • 3Department of Pharmaceutical Chemistry - Division of Drug Synthesis, University of Vienna, Althanstrasse 14, A-1090 Vienna, Austria.

Abstract

The seed mucilage of Hyptis suaveolens L. includes acid - and neutral heteropolysaccharides in a ratio of about 1:1. The anionic charged fraction responsible for swelling and viscous behaviour possesses an average molar mass of Mw=350kg/mol, Mn=255kg/mol. The neutral polysaccharide fraction shows an average molar mass of Mw=47kg/mol and Mn=28kg/mol and is composed of d-Galp-, d-Glcp- and d-Manp residues in a molar ratio of about 3:2:1. The structural features present galactoglucan (30%) and galactoglucomannan (70%) with a high level of terminal β-linked d-Galp residues (18%). Structural details of galactoglucomannan are derived by combined enzymatic and chemical methods as well as NMR spectroscopy. Sequences of octa/nonasaccharide β-d-Glcp-(1→4)[β-d-Galp-(1→2)-α-d-Galp-(1→6)]-β-d-Manp-(1→4)-β-d-Glcp-(1→4)-β-d-Glcp-(1→4)[β-d-Galp-(1→2)-α-d-Galp-(1→6)]-β-d-Manp and lower mass tetrasaccharide repeating units β-d-Glcp-(1→4)[β-d-Galp-(1→2)-α-d-Galp-(1→6)]-β-d-Manp were found. The level of the prebiotic activity is related to the availability of β-linked d-Galp residues in the side chains of the molecules.
Copyright © 2016 Elsevier Ltd. All rights reserved.

KEYWORDS:

Galactoglucan; Galactoglucomannan; Hyptis suaveolens L.; Methylation analysis; Mucilage; NMR spectroscopy; Polysaccharide
PMID:
27979191
DOI:
10.1016/j.foodchem.2016.11.087
[PubMed - in process] 
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