Organogenesis of Abelmoschus moschatus Medik. Using Aseptic Seedling Explants

European Journal of Medicinal Plants, ISSN: 2231-0894,Vol.: 25, Issue.: 1 Short Research Article Aruna Varimadugu1* and Pullaiah Thammineni2 1Department of Biotechnology, Chaitanya Bharathi Institute of Technology, Hyderabad, Telangana, India. 2Department of Botany, Sri Krishnadevaraya University, Anantapur, Andhra Pradesh, India. Article Information Editor(s): (1) Dr. Paolo Zucca, Department of Biomedical Sciences, University of Cagliari, Italy. (2) Dr. Marcello Iriti, Professor, Plant Biology and Pathology, Department of Agricultural and Environmental Sciences, Milan State University, Italy. Reviewers: (1) Claudia Veronica Luna, National University of the Northeast, Argentina. (2) Mahanom Jalil, University of Malaya, Malaysia. (3) Shreeti Pradhan, Tribhuvan University, Nepal. Complete Peer review History: http://www.sciencedomain.org/review-history/26167 Abstracts Aim: Abelmoschus moschatus have been extensively used in traditional medicine as well as in perfume industries. The primary goal of the present research was to develop an efficient plant regeneration protocol of Abelmoschus moschatus from aseptic seedling explants such as cotyledon, internode, leaf and root. Methodology: The seeds of Abelmoschus moschatus were surface sterilized with 0.1% mercuric chloride and 70% ethanol were cultured on ½ MS basal media for developing aseptic seedlings Aseptic seedling explants were cultured on different concentrations of auxins for callus induction. Later callus was transferred on to different concentrations of cytokinins for shoot regeneration and for in vitro, rooting different concentrations of auxins were used. Finally, such in vitro developed plantlets were acclimatized. Results: Half strength MS medium with 1% sucrose was used for raising aseptic seedlings. Maximum of 92% response of callus induction was obtained from leaf explants on MS medium + 2 mg/L 2, 4-dichlorophenoxyacetic acid. An average of 2.4 shoots per callus were observed on MS + 2 mg/L benzyl-6-aminopurine from leaf explant. The regenerated shoots were best rooted on 1/2 MS + 0.5 mg/L indole-3-butyric acid. The regenerated plantlets were established with 70% survival. Conclusion: An efficient plant regeneration protocol of Abelmoschus moschatus was developed. Keywords : Abelmoschus moschatus; callus; leaf explant; shoot regeneration. Full Article - PDF Page 1-8 Article Metrics DOI : 10.9734/EJMP/2018/42720 Review History Comments