Cheryl & ethnovet: Ethnobotanical study, antifungal activity, phytochemical screening and total phenolic content of Algerian Aristolochia longa - Journal of Intercultural Ethnopharmacology - ScopeMed.org

J Intercult Ethnopharmacol. 2014; 3(4): 150-154

doi: 10.5455/jice.20140826030222

Ethnobotanical study, antifungal activity, phytochemical screening and total phenolic content of Algerian Aristolochia longa

Bachir Benarba, Boumedienne Meddah.


Abstract Aim: Aristolochia longa (from the family Aristolochiaceae) is widely used in Algerian traditional medicine. Here we document ethnomedicinal uses by local population of Mascara province (west Algeria) and we evaluate the antifungal activity, the phytochemical composition and total phenolic content of aqueous extract (decoction) of the roots of A. longa from Algeria. Material and Methods: The ethnobotanical investigation was carried out in Mascara Province (West Algeria). Antifungal activity was assessed against Saccharomyces cerevisiae. Total phenolic content was measured using the Folin-Ciocalteu’s reagent. Results: Our results showed that A. longa is widely used to treat several ailments such as cancer (38%), skin infections (14%) and diabetes (11%). Crushed roots are commonly used (89%) mixed with honey, milk, water or other medicinal plants. A. longa aqueous extract induced growth inhibition of S. cerevisiae cells in a dose- and time- dependent manner. An effective suppression of S. cerevisiae (97.06% inhibition of proliferation) was obtained at the 500µg/ml after 72h. Results of the phytochemical screening revealed that A. longa aqueous extract contained various bioactive compounds, including polyphenols and flavonoids. Total phenolic content in A. longa aqueous extract was found to be 6.07±0.12 mg (GAE)/g. Conclusion: A longa may be considered as a promising source of new drugs for treating cancer and as a good antifungal agent. Key words: Aristolochia longa; ethnobotany; phytochemistry; antifungal; phenolic; Mascara; Algeria

REFERENCES

1. Oliveira SG, de Moura F R, Demarco FF, Nascente Pda S, Pino FA,Lund RG. An ethnomedicinal survey on phytotherapy withprofessionals and patients from basic care units in the BrazilianUnified Health System. J Ethnopharmacol 2012;140:428-37. http://dx.doi.org/10.1016/j.jep.2012.01.054 [Pubmed]

2. Asase A, Akwetey GA, Achel DG. Ethnopharmacological use of herbalremedies for the treatment of malaria in the Dangme West Districtof Ghana. J Ethnopharmacol 2010;129:367-76. http://dx.doi.org/10.1016/j.jep.2010.04.001 [Pubmed]

3. Shirwaikar A, Somashekar AP, Udupa AL, Udupa SL, SomashekarS. Wound healing studies of Aristolochia bracteolata Lam.with supportive action of antioxidant enzymes. Phytomedicine2003;10:558-62. http://dx.doi.org/10.1078/094471103322331548 [Pubmed]

4. Alali FQ, Tawaha K, Shehadeh MB, Telfah S. Phytochemical andbiological investigation of Aristolochia maurorum L. Z NaturforschC 2006;61:685-91. [Pubmed]

5. Pacheco AG, Machado de Oliveira P, Pilï¿½-Veloso D, Flï¿½vio de CarvalhoAlcï¿½ntara A 13C-NMR data of diterpenes isolated from Aristolochiaspecies. Molecules 2009;14:1245-62. http://dx.doi.org/10.3390/molecules14031245 [Pubmed]

6. Benarba B, Meddah B. Biological activities of Aristolochia plants:A mini review. J Physiobiochem Metab 2013;2:1-4.

7. Chen YG, Yu LL, Huang R, Liu JC, Lv YP, Zhao Y 3"-hydroxyamentoflavoneand its 7-O-methyl ether, two new biflavonoids from Aristolochiacontorta. Arch Pharm Res 2005;28:1233-5. http://dx.doi.org/10.1007/BF02978204

8. Benarba B, Ambroise G, Aoues A, Meddah B, Vazquez A. Aristolochialonga aqueous extract triggers the mitochondrial pathway ofapoptosis in BL41 Burkitt's lymphoma cells. Int J Green Pharm2012;6:45-9. http://dx.doi.org/10.4103/0973-8258.97128

9. Rajakumar N, Shivanna MB. Ethno-medicinal application ofplants in the eastern region of Shimoga District, Karnataka, India.J Ethnopharmacol 2009;126:64-73. http://dx.doi.org/10.1016/j.jep.2009.08.010 [Pubmed]

10. Trease GE, Evans I. Pharmacognosy. 12th ed. London: Bailliere Tindall;1983. [Pubmed]

11. Kumbhare MR, Guleha V, Sivakumar T. Estimation of total phenoliccontent, cytotoxicity and in-vitro antioxidant activity of stem bark ofMoringa oleifera. Asian Pac J Trop Dis 2012;2012:144-50. http://dx.doi.org/10.1016/S2222-1808(12)60033-4

12. Dhaouadi K, Raboudi F, Estevan C, Barrajï¿½n E, Vilanova E, Hamdaoui M,et al. Cell viability effects and antioxidant and antimicrobial activitiesof Tunisian date syrup (Rub El Tamer) polyphenolic extracts. J AgricFood Chem 2011 12;59:402-6. http://dx.doi.org/10.1021/jf103388m [Pubmed]

13. Assefa B, Glatzel G, Buchmann C. Ethnomedicinal uses of Hageniaabyssinica (Bruce) J.F. Gmel. among rural communities of Ethiopia.J Ethnobiol Ethnomed 2010;6:20. http://dx.doi.org/10.1186/1746-4269-6-20 [Pubmed] [PMC Free Fulltext]

14. Cherif HS, Saidi F, Boutoumi H, Rouibi A, Chaouia C. Identificationand characterization of some chemicals from Aristolochia longa L.Agricultura 2009;3:76-82.

15. Saidi F, Cherif HS, Lazouri H, Aid K, Rouibi A, Bele C, Matea C.Determination of the lipid compounds of Aristolochia Longa L. fromAlgeria. Bull Univ Agric 2009;66:17-23.

16. Saidi F, Cherif HS, Metidji H, Rouibia A, Chaouia C, Abdulhussain MS.In vitro multiplication by direct organogenesis of a medicinal plantAristolochia longa L. Agricultura 2009;3:53-66.

17. Benzakour G, Benkirane N, Amrani M, Oudghiri M. Immunostimulatorypotential of Aristolochia longa L. induced toxicity on liver, intestineand kidney in mice. J Toxicol Environ Health Sci 2011;3:214-22.

18. Alonso-Castro AJ, Villarreal ML, Salazar-Olivo LA, Gomez-Sanchez M,Dominguez F, Garcia-Carranca A. Mexican medicinal plants usedfor cancer treatment: Pharmacological, phytochemical andethnobotanical studies. J Ethnopharmacol 2011;133:945-72. http://dx.doi.org/10.1016/j.jep.2010.11.055 [Pubmed]

19. Ezuruike UF, Prieto JM. The use of plants in the traditionalmanagement of diabetes in Nigeria: Pharmacological andtoxicological considerations. J Ethnopharmacol 2014;155:857-924. http://dx.doi.org/10.1016/j.jep.2014.05.055 [Pubmed]

20. Tebbets B, Yu Z, Stewart D, Zhao LX, Jiang Y, Xu LH, et al. Identificationof antifungal natural products via Saccharomyces cerevisiae bioassay:Insights into macrotetrolide drug spectrum, potency and mode ofaction. Med Mycol 2013;51:280-9. http://dx.doi.org/10.3109/13693786.2012.710917 [Pubmed] [PMC Free Fulltext]

21. Hinou J, Demetzos C, Harvala C, Roussakis C. Cytotoxic andantimicrobial principles from the roots of Aristolochia longa.J Ethnopharmacol 1990;28:149-51.

22. Lans C. Comparison of plants used for skin and stomach problems inTrinidad and Tobago with Asian ethnomedicine. J Ethnobiol Ethnomed2007;3:3. http://dx.doi.org/10.1186/1746-4269-3-3 [Pubmed] [PMC Free Fulltext]

23. Navarro-Garcï¿½a VM, Luna-Herrera J, Rojas-Bribiesca MG, ï¿½lvarez-Fitz P, Rï¿½os MY. Antibacterial activity of Aristolochia brevipes againstmultidrug-resistant Mycobacterium tuberculosis. Molecules2011;16:7357-64. http://dx.doi.org/10.3390/molecules16097357 [Pubmed]

24. Alviano WS, Alviano DS, Diniz CG, Antoniolli AR, Alviano CS,Farias LM, et al. In vitro antioxidant potential of medicinal plantextracts and their activities against oral bacteria based on Brazilianfolk medicine. Arch Oral Biol 2008;53:545-52. http://dx.doi.org/10.1016/j.archoralbio.2007.12.001 [Pubmed]

25. Camporese A, Balick MJ, Arvigo R, Esposito RG, Morsellino N,De Simone F, et al. Screening of anti-bacterial activity of medicinalplants from Belize (Central America). J Ethnopharmacol 2003;87:103-7. http://dx.doi.org/10.1016/S0378-8741(03)00115-6

26. Damiï¿½n-Badillo LM, Salgado-Garciglia R, Martï¿½nez-Muï¿½oz RE,Martï¿½nez-Pacheco MM. Antifungal properties of some Mexicanmedicinal plants. Open Nat Prod J 2008;1:27-33. http://dx.doi.org/10.2174/1874848100801010027

27. Hashemi SR, Zulkifli I, Hair Bejo M, Farida A, Somchit MN. Acutetoxicity study and phytochemical screening of selected herbalaqueous extract in broiler chickens. Int J Pharmacol 2008;4:352-60. http://dx.doi.org/10.3923/ijp.2008.352.360

28. Arif T, Bhosale JD, Kumar N, Mandal TK, Bendre RS, Lavekar GS, et al.Natural products â€“ Antifungal agents derived from plants. J Asian NatProd Res 2009;11:621-38. http://dx.doi.org/10.1080/10286020902942350 [Pubmed]

29. Djeridane A, Yousfi M, Nadjemi B, Maamri S, Djireb F, Stocker P.Phenolic extracts from various Algerian plants as strong inhibitorsof porcine liver carboxylesterase. J Enzyme Inhib Med Chem2006;21:719-26. http://dx.doi.org/10.1080/14756360600810399 [Pubmed]