J Ethnopharmacol. 2016 Sep 29. pii: S0378-8741(16)31040-6. doi: 10.1016/j.jep.2016.09.051. [Epub ahead of print]
In vitro antiprotozoal activity and cytotoxicity of extracts and isolated constituents from Greenwayodendron suaveolens.
Muganza DM1, Fruth B2, Nzunzu JL3, Tuenter E4, Foubert K4, Cos P5, Maes L5, Kanyanga RC6, Exarchou V4, Apers S4, Pieters L4.
Author information
- 1Faculty
of Pharmaceutical Sciences, University of Kinshasa, P.O. Box 212,
Kinshasa XI, Democratic Republic of Congo. Electronic address:
cdmuganza@hotmail.com.
- 2Ludwig Maximilian University
of Munich, Faculty of Biology/ Department Biology II, Großhaderner
Straße 2, D - 82152 Planegg-Martinsried, Germany; Centre for Research
and Conservation / KMDA, Koningin Astridplein 20-26, B - 2018, Antwerp,
Belgium.
- 3Faculty of Pharmaceutical Sciences, University of Kinshasa, P.O. Box 212, Kinshasa XI, Democratic Republic of Congo.
- 4Natural
Products & Food Resarch and Analysis (NatuRA), Department of
Pharmaceutical Sciences, University of Antwerp, Universiteitsplein 1,
B-2610, Antwerp, Belgium.
- 5Laboratory of
Microbiology, Parasitology and Hygiene (LMPH), Faculty of
Pharmaceutical, Biomedical and Veterinary Sciences, University of
Antwerp, Universiteitsplein 1, B-2610, Antwerp, Belgium.
- 6Faculty
of Pharmaceutical Sciences, University of Kinshasa, P.O. Box 212,
Kinshasa XI, Democratic Republic of Congo; Natural Products & Food
Resarch and Analysis (NatuRA), Department of Pharmaceutical Sciences,
University of Antwerp, Universiteitsplein 1, B-2610, Antwerp, Belgium.
- 1Faculty of Pharmaceutical Sciences, University of Kinshasa, P.O. Box 212, Kinshasa XI, Democratic Republic of Congo. Electronic address: cdmuganza@hotmail.com.
- 2Ludwig Maximilian University of Munich, Faculty of Biology/ Department Biology II, Großhaderner Straße 2, D - 82152 Planegg-Martinsried, Germany; Centre for Research and Conservation / KMDA, Koningin Astridplein 20-26, B - 2018, Antwerp, Belgium.
- 3Faculty of Pharmaceutical Sciences, University of Kinshasa, P.O. Box 212, Kinshasa XI, Democratic Republic of Congo.
- 4Natural Products & Food Resarch and Analysis (NatuRA), Department of Pharmaceutical Sciences, University of Antwerp, Universiteitsplein 1, B-2610, Antwerp, Belgium.
- 5Laboratory of Microbiology, Parasitology and Hygiene (LMPH), Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp, Universiteitsplein 1, B-2610, Antwerp, Belgium.
- 6Faculty of Pharmaceutical Sciences, University of Kinshasa, P.O. Box 212, Kinshasa XI, Democratic Republic of Congo; Natural Products & Food Resarch and Analysis (NatuRA), Department of Pharmaceutical Sciences, University of Antwerp, Universiteitsplein 1, B-2610, Antwerp, Belgium.
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE:
The
Nkundo people (Nkundo area of Bolongo, Mai-Ndombe district, Bandundu
Province, DR Congo) use various plant parts of the tree Greenwayodendron
suaveolens (Engl. & Diels) Verdc. (syn. Polyalthia suaveolens Engl.
& Diels) (Annonaceae) against malaria, but its antiprotozoal
constituents are not known.
MATERIALS AND METHODS:
The
crude 80% ethanol extract from the fruits, leaves, root bark and stem
bark and 16 fractions were assessed in vitro for their antiprotozoal
activity against Trypanosoma brucei brucei, T. cruzi, Leishmania
infantum and the chloroquine and pyrimethamine-resistant K1 strain of
Plasmodium falciparum (Pf-K1). Their cytotoxic effects were evaluated
against MRC-5 cells. Active constituents were isolated by
chromatographic means, identified using spectroscopic methods, and
evaluated in the same assays.
RESULTS:
The root bark extract showed the highest activity against P. falciparum K1 (IC50 0.26µg/mL) along with the stem bark alkaloid fraction (IC50 0.27µg/mL). The root bark alkaloid fraction had a pronounced activity against all selected protozoa with IC50
values < 1µg/mL. The 90% methanol fractions of the different plant
parts showed a pronounced activity against P. falciparum K1, with IC50
values ranging between 0.36µg/mL and 0.69µg/mL. Four constituents were
isolated: the triterpenes polycarpol, and dihydropolycarpol, the latter
one being reported for the first time from nature, and the alkaloids
polyalthenol and N-acetyl-polyveoline. They were active to a various
degree against one or more protozoa, mostly accompanied by cytotoxicity.
The highest selectivity was observed for N-acetyl-polyveoline against
P. falciparum K1 (IC50 2.8µM, selectivity index 10.9).
CONCLUSIONS:
These
results may explain at least in part the traditional use of this plant
species against parasitic diseases such as malaria in DR Congo.
Copyright © 2016. Published by Elsevier Ireland Ltd.
Copyright © 2016. Published by Elsevier Ireland Ltd.
