- 1Department of Conservative Dentistry and Periodontology, Poznan University of Medical Sciences, Poznan, Poland.
- 2Department of Cell Biology, Poznan University of Medical Sciences, Poznan, Poland.
- 3Urology Research Center, Department of Urology, College of Medicine, University of Toledo, Toledo, USA.
Abstract
Antiseptic
rinses have been successfully used in inflammatory states of the gums
and oral cavity mucosa. Antibacterial effects of chlorhexidine,
essential oils and some herbs are well documented. Reaction of host
tissue to these substances has much poorer documentation. The aim of the
study was to analyse the influence of chlorhexidine (CHX), essential
oil (EO: thymol, 0.064%; eucalyptol, 0.092%; methyl salicylate, 0.060%;
menthol, 0.042%) mouth rinses and salvia, chamomile and calendula brews
on fibroblast biology in vitro. The human fibroblast CCD16 line cells
were cultured in incubation media which contained the examined
substances. After 24 and 48 hours, the cell morphology, relative growth
and apoptosis were evaluated. Exposure of fibroblasts to CHX, EO or
salvia caused various changes in cell morphology. Cells cultured for 48
hours with CHX revealed a noticeably elongated shape of while cells
cultured in high EO concentration or with salvia were considerably
smaller and contracted with fewer projections. Chlorhexidine, EO and
salvia reduced the fibroblast proliferation rate and stimulated cell
death. Both reactions to EO were dose dependent. Cells exposure to
chamomile or calendula brews did not change morphology or proliferation
of fibroblasts. The results of this in vitro study showed that in
contrast to chamomile and calendula, the brews of EO, CHX or salvia had a
negative influence on fibroblast biology.
KEYWORDS:
chlorhexidine; essential oils; fibroblast; herbs; morphology; mouth rinses; proliferation
