Detection of canine adenovirus 1 in red foxes ( Vulpes vulpes) and raccoons ( Procyon lotor) in Germany with a TaqMan real-time PCR assay.

J Vet Diagn Invest. 2017 Jun 1:1040638717712331. doi: 10.1177/1040638717712331. [Epub ahead of print]

Hechinger S1, Scheffold S1, Hamann HP1, Zschöck M1.

Author information

1

Hessian State Laboratory, Department of Veterinary Medicine, Giessen, Germany.

Abstract

We developed a real-time (rt)PCR assay based on TaqMan probe technology for the specific detection of canine adenovirus 1 (CAdV-1). The assay is able to detect three 50% tissue culture infectious dose/mL in CAdV-1-containing cell culture supernatant. Viral genomes were not amplified of canine adenovirus 2 or of several bovine, porcine, and avian adenoviruses. In silico analysis provided no indication of amplification of other heterologous genomes. The sensitivity of the real-time assay exceeded that of a conventional gel-based CAdV-1 PCR by a factor of 100. Following the integration of the novel PCR into the Hessian wildlife-monitoring program, CAdV-1 DNA was detected in none of the tested raccoons ( n = 48) but in 11 of 97 foxes.

KEYWORDS:

Canine adenovirus; foxes; raccoons; real-time PCR