Lasers Med Sci. 2017 Jun 13. doi: 10.1007/s10103-017-2252-4. [Epub ahead of print]
- 1
- Laboratory of Toxinology and Medicinal Plants, Anhanguera-Uniderp University, Campus III, Street Alexandre Herculano 1400, Campo Grande, MS, 79037-280, Brazil. douradod@uol.com.br.
- 2
- Infectious Diseases, Postgraduate Program, Federal University of Mato Grosso do Sul (UFMS), Campo Grande, MS, 79070-900, Brazil. douradod@uol.com.br.
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- Department of Biochemistry and Tissue Biology, Institute of Biology, State University of Campinas (UNICAMP), Campinas, SP, 13083-863, Brazil. douradod@uol.com.br.
- 4
- Environment and Regional Development, Postgraduate Program, Anhanguera-Uniderp University, Campo Grande, MS, 79037-280, Brazil.
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- Professional Master's Program in Agroindustrial Production and Management, Anhanguera-Uniderp University, Campo Grande, MS, 79037-280, Brazil.
- 6
- Federal University of Piauí; Campus of Parnaíba, Parnaíba, PI, 64202-020, Brazil.
- 7
- Academic of Postgraduate in Biochemistry and Molecular Biology, Federal University of Mato Grosso do Sul (UFMS), Campo Grande, MS, 79070-900, Brazil.
- 8
- Department of Biochemistry and Tissue Biology, Institute of Biology, State University of Campinas (UNICAMP), Campinas, SP, 13083-863, Brazil.
Abstract
Bothropic venom contains a range of biologically active substances capable of causing severe local and systemic envenoming symptomatology within its victims. The snake anti-venom is effective against systemic effects but has no neutralizing effect against the fast developing local effects. Herein, mice gastrocnemius injected with Bothrops moojeni venom (40 μg/kg) or saline solution were irradiated with HeNe (632.8 nm) and GaAs (904 nm) lasers (daily energy density of 4 J/cm2; 0.03/0.21 power density; 0.07/0.16 spot size; 1.2/0.04 total energy, 1 cm off contact, for HeNe and GaAs lasers, respectively) and euthanized in periods ranging from 3 h to 21 days. Blood biochemistry for creatine kinase (CK), alkaline phosphatase (ALP), acid phosphatase (AP), lactate dehydrogenase (LDH), aspartate transaminase (AST), and myoglobin and histopathological analysis, for assessing the degree of myonecrosis and regeneration of gastrocnemius, were done at every time interval. GaAs laser promoted faster photobiomodulation therapy (PBMT) effects, and the GaAs group exhibited a better clinical outcome than the HeNe group. Within the GaAs group, the serum levels of CK, LDH, AP, AST, and myoglobin, which were increased by the physiological effects of the venom, were reduced to initial baseline before snake envenomation in less time than those irradiated by the HeNe laser. However, the group receiving irradiation from the HeNe laser returned the levels of ALP activity to baseline faster than those of the GaAs group. Histopathological analysis revealed enhanced muscle regeneration in mice groups treated with both lasers. PBM promoted by GaAs and HeNe showed well-developed centrally nucleate regenerating cells and an increased number of newly formed blood vessels when compared to unirradiated muscle. We therefore suggest that GaAs had the best outcomes likely derived from a deeper penetrating longer wavelength. We conclude that PMBT is a promising, non-invasive approach to be further tested in pre-clinical studies with a goal to further its clinical use in skeletal muscle recovery in snakebite victims.
KEYWORDS:
Low-level laser irradiation; Myoglobin; Myonecrosis; Photobiomodulation therapy; Viperidae snake
