Sunday, 20 August 2017

Antiproliferative and Antibacterial Activities of Cirsium scabrum from Tunisia.

Evid Based Complement Alternat Med. 2017;2017:7247016. doi: 10.1155/2017/7247016. Epub 2017 Jul 12. Sahli R1,2, Rivière C1, Dufloer C1, Beaufay C3, Neut C4, Bero J3, Hennebelle T1, Roumy V1, Ksouri R2, Quetin-Leclercq J3, Sahpaz S1. Author information 1 Institut Charles Viollette (EA 7394), Université de Lille, 59000 Lille, France. 2 The Laboratory of Aromatic and Medicinal Plants, Biotechnology Centre of Borj-Cédria (CBBC), Hammam-lif, Tunisia. 3 Pharmacognosy Research Group, Louvain Drug Research Institute, Université Catholique de Louvain, Avenue E. Mounier, No. 72, B01.72.03-1200 Brussels, Belgium. 4 UDSL, INSERM U995, UFR Pharmacie, 59000 Lille, France. Abstract Several Cirsium species are known for their uses in traditional medicine and consequently are studied for their phytochemical content and their biological activities. In the framework of a previous study conducted on eight extremophile plants from Tunisia, we highlighted that the crude methanolic extract of C. scabrum, a not investigated thistle, showed moderate but quite selective cytotoxic activity against the cancerous cell line J774 compared to the noncancerous cell line WI38 (IC50 = 11.53 μg/ml on J774, IC50 = 29.89 µg/ml on WI38, and selectivity index = 2.6). In the current study, the partitions of the leaves of C. scabrum were analyzed for their antiproliferative activity on the same cell lines. From the most active petroleum ether partition, we isolated four triterpenoids including lupeol, taraxasterol acetate, and a (1 : 1) mixture of 25-hydroperoxycycloart-23-en-3β-ol and 24-hydroperoxycycloart-25-en-3β-ol. These two cycloartane-type triterpenoids are mostly responsible for this cytotoxic activity. On the other hand, the antimicrobial potential of this plant was also evaluated against 36 microorganisms. The moderate antibacterial activity against 6 Staphylococcus aureus and 2 Dermabacter hominis strains is mainly attributed to the butanol partition whose major compounds are glycosides of flavones. PMID: 28785293 PMCID: PMC5529658 DOI: 10.1155/2017/7247016 Free PMC Article Share on FacebookShare on TwitterShare on Google+ Images from this publication.See all images (1)Free text Figure 1 LinkOut - more resources PubMed Commons home PubMed Commons