Arnica montana L. – a plant of healing: review

Journal of Pharmacy and Pharmacology Volume 69, Issue 8, 1 August 2017, Pages 925-945 Kriplani, P.abEmail Author, Guarve, K.a, Baghael, U.S.c View Correspondence (jump link) aGuru Gobind Singh College of Pharmacy, Yamunanagar, Haryana, India bI.K. Gujral Punjab Technical University, Jalandhar, Punjab, India cDepartment of Pharmaceutical Sciences, Khalsa College, Amritsar, Punjab, India Abstract View references (90) Objectives: Arnica montana is a widely used therapeutic plant used traditionally to treat various ailments. The objective of this study was to evaluate the botany, phytochemistry and ethnopharmacology along with special emphasis given on pharmacological activity of plant A. montana. Key findings: The plant extracts have been reported to possess antibacterial, antitumor, antioxidant, anti-inflammatory, antifungal and immunomodulatory activity. A wide range of chemical compounds including sesquiterpene lactones and their short-chain carbonic acid esters, flavonoids, carotenoids, essential oils, diterpenes, arnidiol, pyrrolizidine alkaloids, coumarins, phenolic acids, lignans and oligosaccharides, etc., are found in different parts of the plant. Summary: It has been scrutinized that extensive research has been carried out to explore the therapeutic potential of flowers of the plant. Therefore, investigations should be carried out to explore the therapeutic potential of other parts of the plant for better therapeutic utilization. © 2017 Royal Pharmaceutical Society Author keywords anti-inflammatory Arnica montana helenalin sesquiterpene lactones Indexed keywords EMTREE drug terms: Arnica montana extractcarotenoidcoumarinditerpeneessential oilesterflavonoidlignanoligosaccharidephenolpyrrolizidine alkaloidsesquiterpene lactoneterpenethymol EMTREE medical terms: analgesic activityantibacterial activityanticoagulationantifungal activityantihair loss activityantiinflammatory activityantimicrobial activityantineoplastic activityantiosteoarthritic activityantiosteoporotic activityantioxidant activityantiplatelet activityArnica montanabiological activitybotanybreathing patterncirculationcoughingdrug activitydrug mechanismethnopharmacologyflowerhair growthhumanhypopigmentationhypopigmentation activityimmunomodulationinotropisminsecticidal activityliver protectionnonhumanossificationphytochemistryplant rootplant seedReviewtherapeutic researchtoxicologyureotonic activity Chemicals and CAS Registry Numbers: coumarin, 91-64-5; phenol, 108-95-2, 3229-70-7; thymol, 89-83-8 ISSN: 00223573 CODEN: JPPMA Source Type: Journal Original language: English DOI: 10.1111/jphp.12724 Document Type: Review Publisher: Blackwell Publishing Ltd References (90) View in search results format All Export Print E-mail Save to PDF Create bibliography View all 90 references 1 Sahid, M., Rao, N.K. First record of the two Asteraceae species from the United Arab Emirates (2015) J New Biol Rep, 4, pp. 215-218. 2 Kenny, O., Smyth, T.J., Walsh, D., Kelleher, C.T., Hewage, C.M., Brunton, N.P. Investigating the potential of under-utilised plants from the Asteraceae family as a source of natural antimicrobial and antioxidant extracts (2014) Food Chemistry, 161, pp. 79-86. Cited 20 times. www.elsevier.com/locate/foodchem doi: 10.1016/j.foodchem.2014.03.126 View at Publisher 3 Obón, C., Rivera, D., Verde, A., Fajardo, J., Valdés, A., Alcaraz, F., Carvalho, A.M. Árnica: A multivariate analysis of the botany and ethnopharmacology of a medicinal plant complex in the Iberian Peninsula and the Balearic Islands (2012) Journal of Ethnopharmacology, 144 (1), pp. 44-56. Cited 11 times. doi: 10.1016/j.jep.2012.08.024 View at Publisher 4 https://en.wikipedia.org/wiki/Asteraceae 5 Nikolić, M., Stevović, S. Family Asteraceae as a sustainable planning tool in phytoremediation and its relevance in urban areas (2015) Urban Forestry and Urban Greening, 14 (4), pp. 782-789. Cited 2 times. http://www.elsevier.com/wps/find/journaldescription.cws_home/701803/description#description doi: 10.1016/j.ufug.2015.08.002 View at Publisher 6 Erbar, C., Leins, P. Diversity of styles and mechanisms of secondary pollen presentation in basal Asteraceae-New insights in phylogeny and function (2015) Flora: Morphology, Distribution, Functional Ecology of Plants, 217, pp. 109-130. Cited 3 times. www.urbanfischer.de/journals/flora/flora.htm doi: 10.1016/j.flora.2015.10.002 View at Publisher 7 Dietz, V. Homeopathic and herbal preparations of Arnica montana for treatment of musculoskeletal injuries (1998) Integrative Medicine, 1, pp. 42-44. 8 Maguire, B. A monograph of the genus arnica - Senecioneae, compositae (1943) Brittonia, 4 (3), pp. 386-510. Cited 35 times. doi: 10.2307/2804900 View at Publisher 9 Aiello, N., Bontempo, R., Vender, C., Ferretti, V., Innocenti, G., Dall'Acqua, S. Morpho-quantitative and qualitative traits of Arnica montana L. wild accessions of Trentino, Italy (2012) Industrial Crops and Products, 40 (1), pp. 199-203. Cited 4 times. doi: 10.1016/j.indcrop.2012.03.013 View at Publisher 10 European Medicines Agency (Science medicines agency), EMA/HMPC/198794/2012, pp. 1-25. 11 Schröder, H., Lösche, W., Strobach, H., Leven, W., Willuhn, G., Till, U., Schrör, K. Helenalin and 11α,13-dihydrohelenalin, two constituents from Arnica montana L., inhibit human platelet function via thiol-dependent pathways (1990) Thrombosis Research, 57 (6), pp. 839-845. Cited 47 times. doi: 10.1016/0049-3848(90)90151-2 View at Publisher 12 Clair, S. Arnica: a proven first aid remedy for injuries and accidents (2010) J N Zeal Assoc Med Herb, pp. 12-13. Cited 2 times. 13 (2000) European Pharmacopoeia, pp. 396-398. Cited 1047 times. 3rd edn, TSO (August 2000), Council of Europe 14 Archer, H.K., Pettit, M.S. (1997) Analgesic and antiphlogistic compositions and therapeutic wrap for topical delivery 15 Alam, M. (2009) Topical Arnica treatment for reducing bruising 16 Marissal, J., Aubert, L.P. (1987) Slenderizing and anti-cellulitis cosmetic composition based on an extract of a plant containing saponins, an extract of Arnica montana L. and a kola nut extract, and to a process for using the same 17 Zelaya, L.M. (1994) Oral rinse composition 18 Paradise, L. Homeopathic pharmaceutical compositions, US 5795573A 19 Dreyer, L.R. Homeopathic formulations useful for treating pain and/or inflammation 20 Bilia, A.R., Bergonzi, M.C., Mazzi, G., Vincieri, F.F. Development and stability of semisolid preparations based on a supercritical CO2 Arnica extract (2006) Journal of Pharmaceutical and Biomedical Analysis, 41 (2), pp. 449-454. Cited 12 times. doi: 10.1016/j.jpba.2005.12.024 View at Publisher 21 (1999) European agency for the evaluation of medicinal products, veterinary medicinal evaluation unit 22 Aeschbach, R., Löliger, J., Scott, B.C., Murcia, A., Butler, J., Halliwell, B., Aruoma, O.I. Antioxidant actions of thymol, carvacrol, 6-gingerol, zingerone and hydroxytyrosol (1994) Food and Chemical Toxicology, 32 (1), pp. 31-36. Cited 496 times. doi: 10.1016/0278-6915(84)90033-4 View at Publisher 23 Petrova, M. Biotechnological approaches for cultivation and enhancement of secondary metabolites in Arnica montana L (2012) Acta Physiol Plant, pp. 123-133. 24 Wagner, H. (1999) Birkhauser Verlag Immunomodulatory agents from plants https//books.google.co.in/books? 25 Ismail, R. Agents for the treatment and protection of the skin 1990 26 Ganzera, M., Egger, C., Zidorn, C., Stuppner, H. Quantitative analysis of flavonoids and phenolic acids in Arnica montana L. by micellar electrokinetic capillary chromatography (2008) Analytica Chimica Acta, 614 (2), pp. 196-200. Cited 46 times. doi: 10.1016/j.aca.2008.03.023 View at Publisher 27 Paßreiter, C.M. Co-occurrence of 2-pyrrolidineacetic acid with the pyrrolizidines tussilaginic acid and isotussilaginic acid and their 1-epimers in Arnica species and Tussilago farfara (1992) Phytochemistry, 31 (12), pp. 4135-4137. Cited 20 times. doi: 10.1016/0031-9422(92)80428-H View at Publisher 28 Staneva, J., Denkova, P., Todorova, M., Evstatieva, L. Quantitative analysis of sesquiterpene lactones in extract of Arnica montana L. by 1H NMR spectroscopy (2011) Journal of Pharmaceutical and Biomedical Analysis, 54 (1), pp. 94-99. Cited 28 times. doi: 10.1016/j.jpba.2010.08.018 View at Publisher 29 Spitaler, R., Schlorhaufer, P.D., Ellmerer, E.P., Merfort, I., Bortenschlager, S., Stuppner, H., Zidorn, C. Altitudinal variation of secondary metabolite profiles in flowering heads of Arnica montana cv. ARBO (2006) Phytochemistry, 67 (4), pp. 409-417. Cited 75 times. doi: 10.1016/j.phytochem.2005.11.018 View at Publisher 30 Spitaler, R., Winkler, A., Lins, I., Yanar, S., Stuppner, H., Zidorn, C. Altitudinal variation of phenolic contents in flowering heads of Arnica montana cv. ARBO: A 3-year comparison (2008) Journal of Chemical Ecology, 34 (3), pp. 369-375. Cited 49 times. doi: 10.1007/s10886-007-9407-x View at Publisher 31 Wagner, S., Merfort, I. Skin penetration behaviour of sesquiterpene lactones from different Arnica preparations using a validated GC-MSD method (2007) Journal of Pharmaceutical and Biomedical Analysis, 43 (1), pp. 32-38. Cited 26 times. doi: 10.1016/j.jpba.2006.06.008 View at Publisher 32 Douglas, J.A., Smallfield, B.M., Burgess, E.J., Perry, N.B., Anderson, R.E., Douglas, M.H., Glennie, V.L. Sesquiterpene lactones in Arnica montana: A rapid analytical method and the effects of flower maturity and simulated mechanical harvesting on quality and yield (2004) Planta Medica, 70 (2), pp. 166-170. Cited 30 times. doi: 10.1055/s-2004-815495 View at Publisher 33 Leven, W., Willuhn, G. Spectrophotometric determination of sesquiterpenelactone S1 in Arnicae Flos Dab 9 with m-dinitro-benzene (1986) Planta Med, 52, pp. 537-538. Cited 8 times. 34 Merfort, I. Caffeoylquinic acids from flowers of Arnica montana and Arnica chamissonis (1992) Phytochemistry, 31 (6), pp. 2111-2113. Cited 92 times. doi: 10.1016/0031-9422(92)80373-M View at Publisher 35 Dziki, U.G. Seeds of Arnica montana and Arnica chamissonis as a potential source of natural antioxidants (2009) Herba Polonica, 55, pp. 60-71. Cited 7 times. 36 Aiello, N., Carlini, A., Fusani, P., Scartezzini, F. Seed yield and germination characteristics of wild accessions of Arnica montana L. from Trentino (Italy) (2014) Journal of Applied Research on Medicinal and Aromatic Plants, 1 (1), pp. 30-33. http://www.journals.elsevier.com/journal-of-applied-research-on-medicinal-and-aromatic-plants/ doi: 10.1016/j.jarmap.2014.03.001 View at Publisher 37 Pljevljakušić, D., Rančić, D., Ristić, M., Vujisić, L., Radanović, D., Dajić-Stevanović, Z. Rhizome and root yield of the cultivated Arnica montana L., chemical composition and histochemical localization of essential oil (2012) Industrial Crops and Products, 39 (1), pp. 177-189. Cited 8 times. doi: 10.1016/j.indcrop.2012.02.030 View at Publisher 38 Kennedy, J.F., Stevenson, D.L., White, C.A., Lombard, A., Buffa, M. Analysis of the oligosaccharides from the roots of Arnica montana L., Artemisia absinthium L., and Artemisia dracuncula L. (1988) Carbohydrate Polymers, 9 (4), pp. 277-285. Cited 4 times. doi: 10.1016/0144-8617(88)90046-X View at Publisher 39 Weremczuk-Jeżyna, I., Wysokińska, H., Kalemba, D. Constituents of the essential oil from hairy roots and plant roots of arnica montana l (2011) Journal of Essential Oil Research, 23 (1), pp. 91-97. Cited 12 times. doi: 10.1080/10412905.2011.9700432 View at Publisher 40 Huber, R., Bross, F., Schempp, C., Gründemann, C. Arnica and stinging nettle for treating burns - A self-experiment (2011) Complementary Therapies in Medicine, 19 (5), pp. 276-280. Cited 7 times. doi: 10.1016/j.ctim.2011.08.001 View at Publisher 41 Lyss, G., Schmidt, T.J., Merfort, I., Pahl, H.L. Helenalin, an anti-inflammatory sesquiterpene lactone from Arnica, selectively inhibits transcription factor NF-κB (1997) Biological Chemistry, 378 (9), pp. 951-961. Cited 166 times. 42 Bellavite, P., Ortolani, R., Conforti, A. Immunology and homeopathy. 3. Experimental studies on animal models (2006) Evidence-based Complementary and Alternative Medicine, 3 (2), pp. 171-186. Cited 43 times. doi: 10.1093/ecam/nel016 View at Publisher 43 Bonamin, L.V., Kawakami, A.P., Sato, C., Cardoso, T.N. Inflammatory process modulation by homeopathic arnica montana 6CH: The role of individual variation (2011) Evidence-based Complementary and Alternative Medicine, 2011, art. no. 917541. Cited 16 times. doi: 10.1155/2011/917541 View at Publisher 44 Widrig, R., Suter, A., Saller, R., Melzer, J. Choosing between NSAID and arnica for topical treatment of hand osteoarthritis in a randomised, double-blind study (2007) Rheumatology International, 27 (6), pp. 585-591. Cited 54 times. doi: 10.1007/s00296-007-0304-y View at Publisher 45 Puhlmann, J., Zenk, M.H., Wagnert, H. Immunologically active polysaccharides of Arnica montana cell cultures (1991) Phytochemistry, 30 (4), pp. 1141-1145. Cited 28 times. doi: 10.1016/S0031-9422(00)95191-4 View at Publisher 46 Koo, H., Gomes, B.P.F.A., Rosalen, P.L., Ambrosano, G.M.B., Park, Y.K., Cury, J.A. In vitro antimicrobial activity of propolis and Arnica montana against oral pathogens (2000) Archives of Oral Biology, 45 (2), pp. 141-148. Cited 229 times. doi: 10.1016/S0003-9969(99)00117-X View at Publisher 47 Sawruk, S. (1995) Method for treatment of osteoporosis 48 Savage, R.H., Roe, P.F. A further double blind trial to assess the benefit of Arnica montana in acute stroke illness (1978) British Homoeopathic Journal, 67 (3), pp. 210-222. Cited 16 times. doi: 10.1016/S0007-0785(78)80008-8 View at Publisher 49 Valan, M.F., Brittob, J.B., Venkataramanc, R. Phytoconstituents with hepatoprotective Activity Int (2010) J Chem Sci, 8, pp. 1421-1432. Cited 10 times. 50 Ahmad, M., Saeed, F., Mehjabeen, Jahan, N. Neuro-pharmacological and analgesic effects of Arnica montana extract (2013) International Journal of Pharmacy and Pharmaceutical Sciences, 5 (4), pp. 590-593. Cited 4 times. http://www.ijppsjournal.com/Vol5Issue4/7780.pdf 51 Keeney, J.A. (2000) Compositions for stimulating hair growth, preventing hair loss, or minimizing hair loss, and methods for preparing and using same 52 de Camargo, R.A., da Costa, E.D., Catisti, R. Effect of the oral administration homeopathic Arnica montana on mitochondrial oxidative stress (2013) Homeopathy, 102 (1), pp. 49-53. Cited 6 times. doi: 10.1016/j.homp.2012.11.001 View at Publisher 53 Marchishin, S.M. Efficacy of phenolic compounds of arnica in toxic liver injury (1983) Farmakologiya i Toksikologiya, 46 (2), pp. 102-106. Cited 2 times. 54 Usui, K., Ikeda, T., Horibe, Y., Nakao, M., Hoshino, T., Mizushima, T. Identification of HSP70-inducing activity in Arnica montana extract and purification and characterization of HSP70-inducers (2015) Journal of Dermatological Science, 78 (1), pp. 67-75. Cited 4 times. www.elsevier.com/locate/jdermsci doi: 10.1016/j.jdermsci.2015.01.014 View at Publisher 55 Kennedy, E.A. (2012) Compositions for stimulating hair growth and preventing hair loss 56 Pawlaczyk, I., Czerchawski, L., Pilecki, W., Lamer-Zarawska, E., Gancarz, R. Polyphenolic-polysaccharide compounds from selected medicinal plants of Asteraceae and Rosaceae families: Chemical characterization and blood anticoagulant activity (2009) Carbohydrate Polymers, 77 (3), pp. 568-575. Cited 50 times. doi: 10.1016/j.carbpol.2009.01.030 View at Publisher 57 Castro, F.C.B., Magre, A., Cherpinski, R., Zelante, P.M., Neves, L.M.G., Esquisatto, M.A.M., Mendonça, F.A.S., (...), Santos, G.M.T. Effects of microcurrent application alone or in combination with topical Hypericum perforatum L. and Arnica montana L. on surgically induced wound healing in Wistar rats (2012) Homeopathy, 101 (3), pp. 147-153. Cited 22 times. doi: 10.1016/j.homp.2012.05.006 View at Publisher 58 Ernst, E. The benefits of Arnica: 16 Case reports (2003) Homeopathy, 92 (4), pp. 217-219. Cited 6 times. doi: 10.1016/j.homp.2003.07.001 View at Publisher 59 Oberbaum, M., Galoyan, N., Lerner-Geva, L., Singer, S.R., Grisaru, S., Shashar, D., Samueloff, A. The effect of the homeopathic remedies Arnica montana and Bellis perennis on mild postpartum bleeding - A randomized, double-blind, placebo-controlled study - Preliminary results (2005) Complementary Therapies in Medicine, 13 (2), pp. 87-90. Cited 25 times. doi: 10.1016/j.ctim.2005.03.006 View at Publisher 60 Duke, J.A. Handbook of Medicinal Plants. Cited 152 times. 2nd edn., 2002, pubished by CRC press 37 61 Woerdenbag, H.J., Merfort, I., Schmidt, T.J., Passreiter, C.M., Willuhn, G., Van Uden, W., Pras, N., (...), Konings, A.W.T. Decreased helenalin-induced cytotoxicity by flavonoids from Arnica as studied in a human lung carcinoma cell line (1995) Phytomedicine, 2 (2), pp. 127-132. Cited 7 times. doi: 10.1016/S0944-7113(11)80057-7 View at Publisher 62 Kaziro, G.S.N. Metronidazole (flagyl) and arnica montana in the prevention of post-surgical complications, a comparative placebo controlled clinical trial (1984) British Journal of Oral and Maxillofacial Surgery, 22 (1), pp. 42-49. Cited 50 times. doi: 10.1016/0266-4356(84)90007-X View at Publisher 63 Albertini, H., Goldberg, W. Evaluation d'un traitement homeopathique de le nevralgie dentaire. Bilan de 60 Observa-Tions Dentaires in Recherches en Homeopathie (1986) Homeopathy, 3, pp. 126-129. 64 Dorfman, P. Évaluation de l'activite de l’Arnica 5CH sur les troubles veineux après perfusion prolongee (1988) Cahiers de Biotherapie, 98, pp. 77-82. Cited 10 times. 65 Lokken, P., Straumsheim, P.A., Tveiten, D., Skjelbred, P., Borchgrevink, C.F. Effect of homoeopathy on pain and other events after acute trauma: Placebo Controlled trial with bilateral oral surgery (1995) BMJ, 310 (6992), p. 1439. Cited 47 times. doi: 10.1136/bmj.310.6992.1439 View at Publisher 66 Hart, O., Mullee, M.A., Lewith, G., Miller, J. Double-blind, placebo-controlled, randomized clinical trial of homoeopathic arnica C30 for pain and infection after total abdominal hysterectomy (1997) Journal of the Royal Society of Medicine, 90 (2), pp. 73-78. Cited 34 times. View at Publisher 67 Tveiten, D., Bruset, S. Effect of Arnica D30 in marathon runners. Pooled results from two double-blind placebo controlled studies (2003) Homeopathy, 92 (4), pp. 187-189. Cited 17 times. doi: 10.1016/j.homp.2003.03.001 View at Publisher 68 Vickers, A.J., Fisher, P., Smith, C., Wyllie, S.E., Rees, R. Homeopathic Arnica 30x is ineffective for muscle soreness after long- distance running: A randomized, double-blind, placebo-controlled trial (1998) Clinical Journal of Pain, 14 (3), pp. 227-231. Cited 53 times. doi: 10.1097/00002508-199809000-00009 View at Publisher 69 Baillargean, L. The effects of Arnica montana on bleeding time. A randomized clinical trial (1998) Biomedical Therapy, 16, pp. 272-276. 70 Ramelet, A.-A., Buchheim, G., Lorenz, P., Imfeld, M. Homoeopathic Arnica in postoperative haematomas: A double-blind study (2000) Dermatology, 201 (4), pp. 347-348. Cited 36 times. doi: 10.1159/000051551 View at Publisher 71 Alonso, D., Lazarus, M.C., Baumann, L. Effects of topical arnica gel on post-laser treatment bruises (2002) Dermatologic Surgery, 28 (8), pp. 686-688. Cited 29 times. doi: 10.1046/j.1524-4725.2002.02011.x View at Publisher 72 Knuesel, O., Weber, M., Suter, A. Arnica montana gel in osteoarthritis of the knee: An open, multicenter clinical trial (2002) Advances in Therapy, 19 (5), pp. 209-218. Cited 55 times. View at Publisher 73 Jeffrey, S.L.A., Belcher, H.J.C.R. Use of Arnica to relieve pain after carpal-tunnel release surgery (2002) Alternative Therapies in Health and Medicine, 8 (2), pp. 66-68. Cited 31 times. 74 Wolf, M., Tamaschke, C., Mayer, W., Heger, M. Efficacy of Arnica in varicose vein surgery: Results of a randomized, double-blind, placebo-controlled pilot trial (2003) Forschende Komplementarmedizin und Klassische Naturheilkunde, 10 (5), pp. 242-247. Cited 6 times. doi: 10.1159/000074778 View at Publisher 75 Stevinson, C., Devaraj, V.S., Fountain-Barber, A., Hawkins, S., Ernst, E. Homeopathic arnica for prevention of pain and bruising: Randomized placebo-controlled trial in hand surgery (2003) Journal of the Royal Society of Medicine, 96 (2), pp. 60-65. Cited 67 times. doi: 10.1258/jrsm.96.2.60 View at Publisher 76 Leivers, K. Unravelling the confusion around arnica's herbal and homoeopathic use (2005) Pharmaceutical Journal, 275 (7365), pp. 289-291. 77 Seeley, B.M., Denton, A.B., Ahn, M.S., Maas, C.S. Effect of homeopathic Arnica montana on bruising in face-lifts: Results of a randomized, double-blind, placebo-controlled clinical trial (2006) Archives of Facial Plastic Surgery, 8 (1), pp. 54-59. Cited 55 times. doi: 10.1001/archfaci.8.1.54 View at Publisher 78 Brinkhaus, B., Wilkens, J.M., Lüdtke, R., Hunger, J., Witt, C.M., Willich, S.N. Homeopathic arnica therapy in patients receiving knee surgery: Results of three randomised double-blind trials (2006) Complementary Therapies in Medicine, 14 (4), pp. 237-246. Cited 19 times. doi: 10.1016/j.ctim.2006.04.004 View at Publisher 79 Robertson, A., Suryanarayanan, R., Banerjee, A. Homeopathic Arnica montana for post-tonsillectomy analgesia: a randomised placebo control trial (2007) Homeopathy, 96 (1), pp. 17-21. Cited 23 times. doi: 10.1016/j.homp.2006.10.005 View at Publisher 80 Totonchi, A., Guyuron, B. A randomized, controlled comparison between arnica and steroids in the management of postrhinoplasty ecchymosis and edema (2007) Plastic and Reconstructive Surgery, 120 (1), pp. 271-274. Cited 49 times. doi: 10.1097/01.prs.0000264397.80585.bd View at Publisher Kriplani, P.; Guru Gobind Singh College of Pharmacy, Yamunanagar, Haryana, India; email:priyanka15n@gmail.com © Copyright 2017 Elsevier B.V., All rights reserved.