Neoboutonia melleri var velutina Prain: in vitro and in vivo hepatoprotective effects of the aqueous stem bark extract on acute hepatitis models

BMC Complement Altern Med. 2018 Jan 22;18(1):24. doi: 10.1186/s12906-018-2091-2. Neoboutonia melleri var velutina Prain: in vitro and in vivo hepatoprotective effects of the aqueous stem bark extract on acute hepatitis models. Endougou Effa AM1,2,3,4, Gantier E5,6, Hennebelle T7, Roumy V7, Rivière C7, Dimo T8, Kamtchouing P8, Desreumaux P5,6,9, Dubuquoy L5,6. Author information 1 Université Lille Nord de France, F-59000, Lille, France. effanmary@gmail.com. 2 Inserm U995, 4e étage Est, Faculté de Médecine - Pôle Recherche, Place Verdun, F-59045, Lille, France. effanmary@gmail.com. 3 Laboratoire de Physiologie Animale, Faculté des Sciences, Université de Yaoundé I, BP, 812, Yaoundé, Cameroon. effanmary@gmail.com. 4 Département de Biologie des Organismes Animaux, Faculté des Sciences, Université de Douala, BP, 24157, Douala, Cameroon. effanmary@gmail.com. 5 Université Lille Nord de France, F-59000, Lille, France. 6 Inserm U995, 4e étage Est, Faculté de Médecine - Pôle Recherche, Place Verdun, F-59045, Lille, France. 7 Laboratoire de pharmacognosie, EA 4481(GRIIOT), Université de Lille 2, Faculté de Pharmacie, F-59006, Lille, France. 8 Laboratoire de Physiologie Animale, Faculté des Sciences, Université de Yaoundé I, BP, 812, Yaoundé, Cameroon. 9 CHU Lille, Service des Maladies de l'Appareil Digestif et de la Nutrition, Hôpital Claude Huriez, F-59037, Lille, France. Abstract BACKGROUND: Hepatitis is a liver inflammation caused by different agents and remains a public health problem worldwide. Medicinal plants are an important source of new molecules being considered for treatment of this disease. Our work aims at evaluating the hepatoprotective properties of Neoboutonia velutina, a Cameroonian medicinal plant. METHODS: The aqueous extract has been prepared using phytochemical methods. HepG2 cells were used to assess anti-inflammatory properties of the extract at different concentrations. Acute hepatitis models (Carbon tetrachloride and Concanavalin A) were performed in mice receiving or not receiving, different extract doses by gavage. Liver injury was assessed using histology, transaminases and pro-inflammatory markers. Extract antioxidant and radical scavenging capacities were evaluated. RESULTS: The extract led to a significant decrease in pro-inflammatory cytokine expression in vitro and to a remarkable protection of mice from carbon tetrachloride-induced liver injury, as shown by a significant decrease in dose-dependent transaminases level. Upon extract treatment, inflammatory markers were significantly decreased and liver injuries were limited as well. In the Concanavalin A model, the extract displayed weak effects. CONCLUSIONS: Taking into account underlying mechanisms in both hepatitis models, we demonstrate the extract's radical scavenging capacity. Neoboutonia velutina displays a potent hepatoprotective effect mediated through radical scavenging properties. KEYWORDS: Acute hepatitis; Antioxidant; Neoboutonia melleri var. velutina Prain; Preventive treatment PMID: 29357846 DOI: 10.1186/s12906-018-2091-2 Free full text https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/s12906-018-2091-2