Oxidative stress and food supplementation with antioxidants in therapy dogs(Article)

Canadian Journal of Veterinary ResearchVolume 81, Issue 3, 2017, Pages 206-216 Sechi, S.a, Fiore, F.a, Chiavolelli, F.c, Dimauro, C.b, Nudda, A.b, Cocco, R.aEmail Author View Correspondence (jump link) aDepartment of Veterinary Medicine, Pathology and Veterinary Clinic Section, University of Sassari, Sassari, Italy bDepartment of Agriculture, Livestock Sciences Section, University of Sassari, Sassari, Italy cDepartment of Diagnostic Medicine, Clinical and Public Health, University of Modena and Reggio Emilia, Modena, Italy Abstract View references (41) The objective of this study was to evaluate the ability of a long-term antioxidant-supplemented diet to regulate the oxidative stress and general health status of dogs involved in animal-assisted intervention (AAI) programs. Oxidative stress is a consequence of the accumulation of reactive oxygen species (ROS). Exercise-induced oxidative stress can increase muscle fatigue and fiber damage and eventually leads to impairment of the immune system. A randomized, placebo-controlled, crossover clinical evaluation was conducted with 11 healthy therapy dogs: 6 females and 5 males of different breeds and with a mean age of 2.7 ± 0.8 y (mean ± SEM). The dogs were divided into 2 groups, 1 fed a high quality commercial diet without antioxidants (CD) and the other a high quality commercial diet supplemented with antioxidants (SD) for 18 wk. After the first 18 wk, metabolic parameters, reactive oxygen metabolite-derivatives (d-ROMs), and biological antioxidant potential (BAP) levels were monitored and showed a significant reduction of d-ROMs, triglycerides, and creatinine values in the SD group (P < 0.05) and a significant increase in amylase values in the CD group (P < 0.01). At the end of this period, groups were crossed over and fed for another 18 wk. A significant decrease in amylase and glutamate pyruvate transaminase (GPT) values was observed in the CD and SD group, respectively (P < 0.05). In conclusion, a controlled, balanced antioxidant diet may be a valid approach to restoring good cell metabolism and neutralizing excess free radicals in therapy dogs. © 2017, Canadian Veterinary Medical Association. All rights reserved. Indexed keywords EMTREE drug terms: antioxidantfatty acidlinolenic acidoleic acidpalmitic acidreactive oxygen metaboliteantioxidant EMTREE medical terms: alanine aminotransferase blood levelanimal experimentArticlecontrolled studycreatinine blood levelcrossover procedurediet supplementationdogfemalemalemetabolomicsnonhumanoxidative stressrandomized controlled trialspectrophotometrytriacylglycerol blood levelanalysisanimalanimal fooddietdietary supplementdrug effectsoxidative stressveterinary MeSH: Animal FeedAnimalsAntioxidantsCross-Over StudiesDietDietary SupplementsDogsFemaleMaleOxidative Stress Chemicals and CAS Registry Numbers: linolenic acid, 1955-33-5, 463-40-1; oleic acid, 112-80-1, 115-06-0; palmitic acid, 57-10-3; Antioxidants ISSN: 08309000 CODEN: CJVRE Source Type: Journal Original language: English PubMed ID: 28725111 Document Type: Article Publisher: Canadian Veterinary Medical Association References (41) View in search results format All 1 Mantovani, G., Macciò, A., Madeddu, C., Mura, L., Gramignano, G., Lusso, M.R., Mulas, C., (...), Elsener, B. 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