EFFECT OF CARISSA CARANDAS ON CLINICO-HAEMATOLOGICAL PROFILE ON MONOCROTOPHOS TOXICITY IN RATS

OMICS journal Antidiabetic effect of Carissa carandas in rats and the mechanism of its insulin secretagogues activity in isolated pancreatic islets 5th International Conference and Exhibition on Pharmacology and Ethnopharmacology Rambir Singh and Poonam Sharma Bundelkhand University, India Indira Gandhi National Tribal University, India Posters & Accepted Abstracts: Clin Exp Pharmacol DOI: 10.4172/2161-1459.C1.017 Abstract Carissa Carandas (CC) has been documented as a traditional treatment for diabetes. In the present study, the CC fruit aqueous, methanol, chloroform and ethyl acetate extracts were examined for hypoglycemic activity in healthy Wistar rats. Aqueous extract of CC (AECC) showed highest fall of 67.08% in fasting blood glucose from 0 to 1h in Glucose Tolerance Test (GTT). The ED50 of AECC was 300 mg/kgbw in streptozotocin induced diabetic rats. Treatment of diabetic rats with ED50 of AECC for 28 days significantly reduced post prandial glucose (PPG) by 33.65% (p<0.01), glycosylated hemoglobin (HbA1c) by 45.79% (p<0.01) and increased insulin level by 69.7% (p<0.05). The increase in insulin secretion may be partly responsible for antidiabetic effect of AECC. To assess the mechanism of secretagogues activity, AECC was incubated with isolated pancreatic islets of Wistar rats at basal (3.3 mM) and high (16.7 mM) level of glucose in presence or absence of diazoxide (K-ATP channel opener), nimodipine (Ca2+ Channel blocker) and calphostin-C (PKC inhibitor). AECC induced insulin secretion at 16.7 mM of glucose was significantly (p<0.01) reduced by diazoxide and nimodipine, but non-significantly (p>0.05) by calphostin-C. The study indicated that the phytochemicals present in AECC may be inducing insulin secretion by closing K-ATP channels in β-cells of pancreatic islets. Biography Email: sehrawat_r@yahoo.com