Glycyrrhiza glabra HPLC fractions: identification of Aldehydo Isoophiopogonone and Liquirtigenin having activity against multidrug resistant bacteria.

Format: Abstract Send to BMC Complement Altern Med. 2018 May 2;18(1):140. doi: 10.1186/s12906-018-2207-8. Rahman H1, Khan I2, Hussain A3, Shahat AA4,5, Tawab A6, Qasim M2, Adnan M7, Al-Said MS4, Ullah R4, Khan SN8. Author information 1 Department of Microbiology, Abdul Wali Khan University Mardan, Mardan, Pakistan. hazirrahman@hotmail.com. 2 Department of Microbiology, Kohat University of Science & Technology, Kohat, Pakistan. 3 Department of Botany, Abdul Wali Khan University, Mardan, Pakistan. 4 Department of Pharmacognosy and Medicinal Aromatic, and Poisonous Plants Research Center, College of Pharmacy King Saud University, Riyadh, 11451, Saudi Arabia. 5 Phytochemistry Department, National Research Centre, P.O. Box 12622, 33 El Bohouth st., Dokki, Giza, Egypt. 6 National Institute for Biotechnology and Genetic Engineering, Faisalabad, Pakistan. 7 Department of Botany, Kohat University of Science & Technology, Kohat, Pakistan. 8 Department of Zoology, Kohat University of Science & Technology, Kohat, Pakistan. Abstract BACKGROUND: Medicinal plants have been founded as traditional herbal medicine worldwide. Most of the plant's therapeutic properties are due to the presence of secondary metabolites such as alkaloids, glycosides, tannins and volatile oil. METHODS: The present investigation analyzed the High-Pressure Liquid Chromatography (HPLC) fractions of Glycyrrhiza glabra (Aqueous, Chloroform, Ethanol and Hexane) against multidrug resistant human bacterial pathogens (Escherichia coli, Acinetobacter baumannii, Staphylococcus aureus and Pseudomonas aeruginosa). All the fractions showed antibacterial activity, were subjected to LC MS/MS analysis for identification of bioactive compounds. RESULTS: Among total HPLC fractions of G. glabra (n = 20), three HPLC fractions showed potential activity against multidrug resistant (MDR) bacterial isolates. Fraction 1 (F1) of aqueous extracts, showed activity against A. baumannii (15 ± 0.5 mm). F4 from hexane extract of G. glabra showed activity against S. aureus (10 ± 0.2 mm). However, F2 from ethanol extract exhibited activity against S. aureus (10 ± 0.3 mm). These active fractions were further processed by LC MS/MS analysis for the identification of compounds. Ellagic acid was identified in the F1 of aqueous extract while 6-aldehydo-isoophiopogonone was present in F4 of hexane extract. Similarly, Liquirtigenin was identified in F2 of ethanol. CONCLUSIONS: Glycyrrhiza glabra extracts HPLC fractions showed anti-MDR activity. Three bioactive compounds were identified in the study. 6-aldehydo-isoophiopogonone and Liquirtigenin were for the first time reported in G. glabra. Further characterization of the identified compounds will be helpful for possible therapeutic uses against infectious diseases caused by multidrug resistant bacteria. KEYWORDS: Anti-MDR activity; Ellagic acid 6-aldehydo-isoophiopogonone; Glycyrrhiza glabra; HPLC fractionation; Liquirtigenin PMID: 29720152 PMCID: PMC5930497 DOI: 10.1186/s12906-018-2207-8 [Indexed for MEDLINE] Free PMC Article Images from this publication.See all images (3)Free text Fig. 1