Anthelmintic activity of Tunisian chamomile (Matricaria recutita L.) against Haemonchus contortus

Journal of HelminthologyVolume 92, Issue 2, 1 March 2018, Pages 168-177 Hajaji, S.a,bEmail Author, Alimi, D.a,b, Jabri, M.A.b,c, Abuseir, S.d, Gharbi, M.a, Akkari, H.a aLaboratoire de Parasitologie, Université de la Manouba, École Nationale de Médecine Vétérinaire de Sidi Thabet, 2020, Tunisia bFaculté des Sciences de Bizerte, Université de Carthage, Zarzouna, 7021, Tunisia cLaboratoire de Physiologie Intégrée, Faculté des Sciences de Bizerte, Département des Sciences de la Vie, Zarzouna, 7021, Tunisia View additional affiliations Abstract View references (65) The chemical treatment of gastrointestinal parasitic diseases has been undermined by increasing resistance and high toxicity. There is an urgent need to search for alternative natural sources for the treatment of such parasites. In this respect, the present study aims to quantify phenolic compounds of chamomile (Matricaria recutita L.) and to study their in vitro anti-oxidant and anthelmintic activities in solvents with increasing polarity. In vitro determination of anti-oxidant capacity was carried out using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-azino-bis-(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) radical cation methods. In vitro anthelmintic activity was investigated on egg-hatching inhibition and loss of motility of adult worms of Haemonchus contortus from sheep. The results showed that methanolic and aqueous extracts contain more total polyphenols, total flavonoids and condensed tannins than chloroformic and hexanic extracts. ABTS and DPPH assays showed that methanolic extracts had the highest anti-oxidant potency (IC50 = 1.19 μg/ml and 1.18 μg/ml, respectively). In vitro anthelmintic activity showed that both methanolic (IC50 = 1.559 mg/ml) and aqueous (IC50 = 2.559 mg/ml) extracts had the greatest effect on egg hatching and motility of worms (100% after 8 h post exposure at 8 mg/ml). A significant and positive correlation between DPPH and ABTS tests was observed for all tested extracts. Therefore, total phenolic, total flavonoid and condensed tannin values were correlated with IC50 from both ABTS and DPPH, and with inhibition of egg hatching. To our knowledge, this report is the first of its kind to deal with in vitro anthelmintic activities of chamomile extracts. © Copyright Cambridge University Press 2017. SciVal Topic Prominence Topic: Anthelmintics | Albendazole | vitro anthelmintic Prominence percentile: 59.766 ISSN: 0022149X CODEN: JOHLA Source Type: Journal Original language: English DOI: 10.1017/S0022149X17000396 Document Type: Article Publisher: Cambridge University Press References (65) View in search results format All 1 Akhtar, M.S., Iqbal, Z., Khan, M.N., Lateef, M. Anthelmintic activity of medicinal plants with particular reference to their use in animals in the Indo-Pakistan subcontinent (2000) Small Ruminant Research, 38 (2), pp. 99-107. Cited 156 times. doi: 10.1016/S0921-4488(00)00163-2 View at Publisher 2 Akkari, H., Jebali, J., Gharbi, M., Mhadhbi, M., Awadi, S., Darghouth, M.A. Epidemiological study of sympatric Haemonchus species and genetic characterization of Haemonchus contortus in domestic ruminants in Tunisia (2013) Veterinary Parasitology, 193 (1-3), pp. 118-125. 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