Antibacterial and antibiotic modifying activity evaluation of ruminants' body fat used as zootherapeutics in ethnoveterinary practices in Northeast Brazil

Journal of Ethnopharmacology

Volume 233, 6 April 2019, Pages 87-93

Author links open overlay panelDiógenesde Queiroz Dias^a

Débora LimaSales^aJacqueline CosmoAndrade^aAna Raquel Pereirada Silva^bSaulo RelisonTintino^bCícera Datiane de MoraisOliveira-Tintino^bGyllyandesonde Araújo Delmondes^bMaysade Oliveira Barbosa^aHenrique Douglas MeloCoutinho^bFelipe SilvaFerreira^cMarcos Fábio GadelhaRocha^dDaniela Maria do Amaral FerrazNavarro^eSuyana Karolyne Linoda Rocha^eJosé Galberto Martinsda Costa^bRomulo Romeu da NóbregaAlves^fWaltécio de OliveiraAlmeida^b

^a

Universidade Federal Rural de Pernambuco – UFRPE, Recife, PE, Brazil

^b

Universidade Regional do Cariri – URCA, Crato, CE, Brazil

^c

Universidade Federal do Vale do São Francisco – UNIVASF, Senhor do Bomfim, BA, Brazil

^d

Universidade Estadual do Ceará – UECE, Fortaleza, CE, Brazil

^e

Universidade Federal de Pernambuco – UFPE, Recife, PE, Brazil

^f

Universidade Estadual da Paraíba – UEPB, Campina Grande, PB, Brazil

Received 9 November 2018, Revised 7 December 2018, Accepted 8 December 2018, Available online 25 December 2018.

https://doi.org/10.1016/j.jep.2018.12.012Get rights and content

Abstract

Ethnopharmacological relevance

Northeast Brazilian ethnoveterinary studies associated with the medicinal use of zootherapies have shown that ruminants’ body fat such as sheep (Ovis aries), goats (Capra hircus) and cows (Bos taurus) are used in diseases affecting domestic animals.

Aim of the study

The objective of this study was to evaluate the antibacterial activity of the fixed oils from these ruminants in isolation and in association with antibiotics.

Results

Ovis aries (OFOA), Capra hircus (OFCH) and Bos taurus (OFBT) fixed oils were extracted using a Soxhlet apparatus with hexane as the solvent. Through the use of gas chromatography coupled to mass spectrometry (GC-MS) the methyl esters from the ruminants’ fixed oils were obtained and the fatty acids present in these oils were indirectly determined. The OFOA, OFCH and OFBT antibacterial and antibiotic modifying activities against standard and multi-resistant bacterial strains were carried out using the broth microdilution test. The fixed oils from these species did not present antibacterial activity when tested in isolation, obtaining Minimal Inhibitory Concentration (MICs) values ≥ 1024 μg/mL. However, when associated with antibiotics, OFBT and OFCH showed a synergistic activity for the Amicacin, Amoxicillin, Norfloxacin and Oxytetracycline antibiotics.

Conclusion

The OFOA promoted a synergistic action for the same antibiotics with the exception of Norfloxacin.