Effects of Nigella sativa on immune responses and pathogenesis of avian influenza (H9N2) virus in turkeys

Journal of Applied Poultry ResearchVolume 25, Issue 1, 6 November 2015, Pages 95-103

(Article)

• Umar, S.abEmail Author,
 
• Rehman, A.c,
 
• Younus, M.d,
 
• Qamar-Un-Nisad,
 
• Ali, A.d,
 
• Shahzad, M.d,
 
• Shah, M.A.A.a,
 
• Munir, M.T.a,
 
• Aslam, H.B.e,
 
• Yaqoob, M.f
 
• View Correspondence (jump link)

• aDepartment of Pathobiology, University of Arid Agriculture, Rawalpindi, Pakistan
• bNational Veterinary School, Toulouse, France
• cFriedrich-Loeffler-Institute of Epidemiology, Berlin, Germany

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Abstract View references (38)

The main objective of this study was to determine the effects of various levels of dietary Nigella sativa seeds on immune-responsiveness and pathogenesis of H9N2 avian influenza virus (AIV) in Turkeys. The experiment was performed on 80 unvaccinated Turkey poults, divided into four groups of 20 birds each. Group A was kept as a non-infected, non-treated negative control; group B was inoculated intranasally with H9N2 AIV (106 EID50) at 4 wk of age; groups C and D were fed on diets containing 1% and 3% Nigella sativa seeds, respectively, from d 1 through the entire experiment period and inoculated intranasally with H9N2 AIV (106 EID50) at the wk 4 of age. Infected birds showed clinical signs of differing severity, with the most prominent disease signs appearing in birds of the H9N2 group. All infected birds showed positive results for virus shedding; however, the pattern of virus shedding was different, with birds of group B showing more pronounced virus secretion than the birds in the groups C and D, which were fed different levels of N. sativa. Moreover, significantly higher antibody titer against H9N2 AIV in group D birds show the immunomodulatory nature of N. sativa. Similarly, increased IFN-γ mRNA expression suggest antiviral behavior of N. sativa, leading to suppressed pathogenesis of H9N2 viruses in N. sativa-fed birds.This study showed that dietary supplementation with N. sativa seed at the level of 3% would enhance immune responsiveness and suppress pathogenicity of influenza viruses in Turkeys. © 2016 2015 Poultry Science Association Inc.

Author keywords

• avian influenza
 
• H9N2
 
• Nigella sativa
 
• Turkey

• ISSN: 10566171
• Source Type: Journal
• Original language: English

• DOI: 10.3382/japr/pfv070
• Document Type: Article
• Publisher: Oxford University Press

• References (38)

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  Umar, S.; Department of Pathobiology, University of Arid Agriculture, Rawalpindi, Pakistan; email:s.umar@envt.fr 
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