HPTLC determination of diosgenin in fenugreek seeds.

Acta Pharm. 2018 Mar 1;68(1):97-107. doi: 10.2478/acph-2018-0002. Król-Kogus B1, Lamine KM2, Migas P1, Boudjeniba M2, Krauze-Baranowska M1. Author information 1 1Department of Pharmacognosy with Medicinal Plants Garden Medical University of Gdańsk 80-416 Gdańsk, Poland. 2 2Laboratoire d'Ethnobotanique et Substances Naturelles Ecole Normale Supérieure de Kouba B.P 92 Kouba, 16050 Algerie. Abstract A new HPTLC-densitometric method for diosgenin determination in fenugreek seeds was established after optimization of the conditions for efficient saponin extraction and acid hydrolysis. Several procedures were tested, the best of which was a three-step Soxhlet extraction, followed by hydrolysis of the obtained methanolic extract with 2 mol L-1 H2SO4. Best diosgenin separation from other hydrolysis products was obtained on HPTLC Si60F254 plates u sing a mixture of n-heptane/ethyl acetate (7:3, V/V) and modified anisaldehyde as a spraying reagent. The method was preliminarily validated and the determined amounts of diosgenin in fenugreek seeds of Polish and African origin were found to be similar and ranged from 0.12-0.18 %. KEYWORDS: HPTLC; diosgenin; fenugreek (Trigonella foenum-graecum); quantitative analysis PMID: 29453912 DOI: 10.2478/acph-2018-0002