Proliferative and antioxidant activity of Symphytum officinale root extract.

Nat Prod Res. 2018 Mar;32(5):605-609. doi: 10.1080/14786419.2017.1326492. Epub 2017 May 10. Sowa I1, Paduch R2,3, Strzemski M1, Zielińska S4, Rydzik-Strzemska E1, Sawicki J1, Kocjan R1, Polkowski J1, Matkowski A4, Latalski M5, Wójciak-Kosior M1. Author information 1 a Department of Analytical Chemistry , Medical University of Lublin , Lublin , Poland. 2 b Faculty of Biology and Biotechnology, Department of Virology and Immunology , Maria Curie-Sklodowska University , Lublin , Poland. 3 c Department of General Ophthalmology , Medical University of Lublin , Lublin , Poland. 4 d Department of Pharmaceutical Biology , Wroclaw Medical University , Wroclaw , Poland. 5 e Children's Orthopedics Department , Medical University of Lublin , Lublin , Poland. Abstract The root of Symphytum officinale L. is commonly used in folk medicine to promote the wound healing, reduce the inflammation and in the treatment of broken bones. The objective of our investigation was to analyse the extract from S. officinale in term of its antioxidant activity and the effect on cell viability and proliferation of human skin fibroblast (HSF). Moreover, the quantification of main phenolics and allantoin was conducted using HPLC-DAD method. Five compounds were found: rosmarinic, p-hydroxybenzoic, caffeic, chlorogenic and p-coumaric acid. DPPH, FRAP and TPC assay showed the high antioxidant activity of the extract. MTT test proved the stimulatory effect on cell metabolism and viability of HSF cells. Moreover, no changes in cytoskeleton structure and cells shape were observed. The obtained results indicate that non-toxic extract from S. officinale root has strong antioxidant potential and a beneficial effect on human skin fibroblasts. KEYWORDS: Symphytum officinale; allantoin; antioxidant activity; cell viability; comfrey; human skin fibroblast; phenolic acids PMID: 28490191 DOI: 10.1080/14786419.2017.1326492