Dynamics of Ca2+ sensitivity of nonpregnant and pregnant buffalo myometrium.

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=1&cad=rja&uact=8&ved=0ahUKEwj3lZbh0MHbAhXaHjQIHaZJA7YQFgguMAA&url=https%3A%2F%2Fwww.researchgate.net%2Fpublication%2F281436313_Dynamics_of_Ca2_sensitivity_of_nonpregnant_and_pregnant_buffalo_myometrium&usg=AOvVaw13oT6N7ska_i3NlayNHmfk January 2014 Project: phd from mathura.. Udayraj P. NakadeUdayraj P. NakadeAbhishek SharmaAbhishek SharmaSoumen ChoudhurySoumen ChoudhurySatish Kumar GargSatish Kumar Garg Abstract This study was proposed to delineate the physiological effects of calcium on gravid and non gravid myometrium of buffaloes. CaCl2 was used to study the differential tonicity of buffalo myometrium isolated from non pregnant, early and late pregnancy stage buffaloes. Results revealed that, removal of extracellular calcium completely inhibited the normal rhythmic spontaneity of buffalo myometrium. Ca2+ produced dosedependant contractile effect on buffalo myometrium in Ca2+ free RLS in nonpregnant, early and late stages of pregnancy. The pD2 and the maximal efficacy (Emax) values produced by CaCl2 were 1.92 ± 0.33, 2.38 ± 0.44 g, (n = 6); 2.20 ± 0.34, 1.45 ± 0.34 (n=6); 2.34 ± 0.17, 4.10 ± 0.61(n=6) in nonpregnant, earlypregnant and latepregnant buffalo myometrium, respectively. From the study, it was concluded that myometrium of early pregnant uterus is least sensitive to extracellular calcium, presumably render the tissue less excitable, whereas, in late pregnancy, myometrium exhibited increased sensitivity to extracellular Ca2+