Isolation, preliminary structural characterization and hypolipidemic effect of polysaccharide fractions from Fortunella margarita (Lour.) Swingle

doi:10.1016/j.foodhyd.2015.05.028

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Highlights

•: FMPS1, FMPS2, FMPS3 and FMPS4 were isolated by column chromatography.
•: Polysaccharide fractions displayed different structure and hypolipidemic effect.
•: The multiple relations between structure and hypolipidemic effect were investigated.
•: The hypolipidemic effect was a combinational result of multiple structural factors.

Abstact

In order to understand the multiple relationships between the structural characteristics and hypolipidemic effect of polysaccharides from Fortunella margarita (Lour.) Swingle (FMPS), four polysaccharide fractions, FMPS1, FMPS2, FMPS3 and FMPS4, were isolated from FMPS by the column chromatography using DEAE Sepharose CL-6B and Sephadex G-100. The results showed that FMPS1 and FMPS3 were mainly pectic polysaccharides while FMPS2 and FMPS4 were mainly composed of neutral polysaccharides. FMPS1, FMPS2 and FMPS3 linked by α- and β-glycosidic linkages while FMPS4 only linked by β-glycosidic linkages. According to the size-exclusion chromatography coupled with multi-angle laser light-scattering and refractive index (SEC-MALLS-RI) system, the molecular weight of FMPS1 and FMPS2 was greater compared to FMPS3 and FMPS4. The chain conformation analysis indicated FMPS3 and FMPS4 were highly branched polymers whereas FMPS1 and FMPS2 existed as globular shape and random coil conformation in aqueous, respectively. In addition, among the four polysaccharide fractions, FMPS1 and FMPS3 displayed the stronger inhibitory effect on the pancreatic lipase, FMPS1 and FMPS2 displayed the stronger ability to bind bile acids, and FMPS3 and FMPS4 displayed the greater antioxidant activity. The hypolipidemic activity of polysaccharide fractions was affected by the monosaccharide composition, glycosidic linkage, molecular weight and chain conformation in aqueous. This functional property was a result of a combination of multiple structural factors.

Graphical abstract

Keywords

Fortunella margarita (Lour.) Swingle;
Polysaccharide fractions;
Preliminary structural characterization;
Hypolipidemic effect

: Corresponding author. College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China. Tel.: +86 591 83736738; fax: +86 591 83739118.

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Friday, 28 August 2015