Thursday, 26 November 2015

2013 Antiviral properties of herbal flavonoids - Inhibitors of the DNA and RNA synthesis

Volume 29, Issue 2, 2013, Pages 150-156

Institute of Molecular Biology and Genetics, NAS of Ukraine, 150, Akademika Zabolotnoho Str, Kyiv, Ukraine
Gromashevsky L. V. Institute of Epidemiology and Infectious Diseases, NAMS of Ukraine, 5, Amosova Str, Kyiv, Ukraine


Aim. To investigate influence of the Deschampsia caespitosa L. and Calamagrostis epogeios L. herbal extract (HE) and its main components -quercetin derivatives -on the DNA and RNA synthesis; to perform comparative analysis of their antiviral activities. Methods. The model transcription system of bacteriophage T7 (RNAP T7) and polymerase chain reaction (PCR) were used as test systems. For antiviral studies there were employed models of influenzal and herpetic infections and hepatitis C. Results. It was shown, that HE, its major component 7, 3'-dimetoxyquercetin (DMQ) its minor component 5,7,3',4'-tetrametho-xyqeercetin (TMQ) effectively inhibited the RNA synthesis in the RNAP T7 system with the corresponding values of IC50-0.07, 4 and 1 µg/ml. Minimal concentrations of the agents IC90, completely inhibiting PCR, were-8, 30 and 40 µg/ml for HE, TMQ and DMQ, accordingly. All the studied preparations revealed high multiple antiviral activities against the RNA-and DNA-containing viruses. Conclusions. Antiviral activity of the herbal extract is likely determined by the combined action of its components, TMQ and DMQ including. Bearing in mind multi-target profile of quercetin derivatives, an assumption may be made, that antiviral mechanism of the agents studied consists in blocking of enzymatic systems of the RNA and DNA synthesis. © Institute of Molecular Biology and Genetics, NAS of Ukraine, 2013.

Author keywords

Antiviral activity; Herbal quercetin derivatives; Inhibitors of RNA and DNA synthesis

Indexed keywords

EMTREE drug terms: Calamagrostis epogeios extract; Deschampsia caespitosa extract; flavonoid; plant extract; unclassified drug
EMTREE medical terms: antiviral activity; article; bacteriophage T7; DNA synthesis; hepatitis C; IC 50; influenza; nonhuman; polymerase chain reaction; RNA synthesis
Species Index: Calamagrostis; Deschampsia cespitosa
ISSN: 02337657Source Type: Journal Original language: Ukrainian
DOI: 10.7124/bc.000813Document Type: Article
Publisher: National Academy of Sciences of Ukraine