Volume 29, Issue 2, 2013, Pages 150-156
a
Institute of Molecular Biology and Genetics, NAS of Ukraine, 150, Akademika Zabolotnoho Str, Kyiv, Ukraine
b Gromashevsky L. V. Institute of Epidemiology and Infectious Diseases, NAMS of Ukraine, 5, Amosova Str, Kyiv, Ukraine
b Gromashevsky L. V. Institute of Epidemiology and Infectious Diseases, NAMS of Ukraine, 5, Amosova Str, Kyiv, Ukraine
Abstract
Aim. To investigate influence of the Deschampsia caespitosa L. and Calamagrostis epogeios L. herbal
extract (HE) and its main components -quercetin derivatives -on the DNA
and RNA synthesis; to perform comparative analysis of their antiviral
activities. Methods. The model transcription system of bacteriophage T7
(RNAP T7) and polymerase chain reaction (PCR) were used as test systems.
For antiviral studies there were employed models of influenzal and
herpetic infections and hepatitis C. Results. It was shown, that HE, its
major component 7, 3'-dimetoxyquercetin (DMQ) its minor component
5,7,3',4'-tetrametho-xyqeercetin (TMQ) effectively inhibited the RNA
synthesis in the RNAP T7 system with the corresponding values of
IC50-0.07, 4 and 1 µg/ml. Minimal concentrations of the agents IC90,
completely inhibiting PCR, were-8, 30 and 40 µg/ml for HE, TMQ and DMQ,
accordingly. All the studied preparations revealed high multiple
antiviral activities against the RNA-and DNA-containing viruses.
Conclusions. Antiviral activity of the herbal
extract is likely determined by the combined action of its components,
TMQ and DMQ including. Bearing in mind multi-target profile of quercetin
derivatives, an assumption may be made, that antiviral mechanism of the
agents studied consists in blocking of enzymatic systems of the RNA and
DNA synthesis. © Institute of Molecular Biology and Genetics, NAS of Ukraine, 2013.
Author keywords
Antiviral activity; Herbal quercetin derivatives; Inhibitors of RNA and DNA synthesis
Indexed keywords
EMTREE drug terms: Calamagrostis epogeios extract; Deschampsia caespitosa extract; flavonoid; plant extract; unclassified drug
EMTREE medical terms: antiviral activity; article;
bacteriophage T7; DNA synthesis; hepatitis C; IC 50; influenza;
nonhuman; polymerase chain reaction; RNA synthesis
Species Index: Calamagrostis; Deschampsia cespitosa
ISSN: 02337657Source Type: Journal
Original language: Ukrainian
DOI: 10.7124/bc.000813Document Type: Article
Publisher: National Academy of Sciences of Ukraine
