Thursday, 26 November 2015

Intraspecific variability in the content of phenolic compounds, essential oil and mucilage of small-leaved lime (Tilia cordata Mill.) from Poland

industrial Crops and Products
Volume 78, December 30, 2015, Pages 58-65

Laboratory of New Herbal Products, Department of Vegetable and Medicinal Plants, Faculty of Horticulture, Biotechnology and Landscape Architecture, Warsaw University of Life Sciences SGGW, 159 Nowoursynowska Street, Warsaw, Poland
Division of Food Quality Evaluation, Department of Biotechnology and Microbiology, Faculty of Food Sciences, Warsaw University of Life Sciences SGGW, 159 Nowoursynowska Street, Warsaw, Poland


The aim of the study was to investigate the chemical variability of small-leaved lime (Tilia cordata Mill.) growing wild in the eastern area of Poland, in terms of content and composition of phenolic compounds (phenolic acids and flavonoids), composition of essential oil and content of mucilage in flowers. Analyzed populations differed significantly when regards the accumulation of biologically active compounds. The total content of phenolic acids ranged from 0.17 to 1.87g×100g-1, flavonoids from 0.09 to 0.52g×100g-1, and mucilage from 0.6 to 3.0g×100g-1. Using validated HPLC-DAD method, the chemical composition of phenolic acids and flavonoids were determined. Among phenolic acids, four compounds were identified with vanillic acid as the dominant one (391.5-1718.0mg×100g-1). Among flavonoids, nine compounds were found. Rutoside and (-)-epicatechin (tannin precursor) were present in the highest amounts (1264.1-4654.6mg×100g-1 and 1000.3-4745.6mg×100g-1, respectively). GC-MS and GC-FID were used to perform the qualitative and quantitative analyze of the essential oil. The major compounds were alkanes: tricosane (5.7-20.7%), pentacosane (3.0-6.1%) and heneicosane (1.1-6.1%), as well as acetophenon (3.5-6.9%), nonanal (1.3-7.8%) and nonanoic acid (3.9-18.2%). © 2015 Elsevier B.V.

Author keywords

Essential oil; Flavonoids; Intraspecific variability; Mucilage; Phenolic acids; Tilia cordata

Indexed keywords

Engineering controlled terms: Adhesives; Bioactivity; Chemical analysis; Flavonoids; Lime; Organic acids; Phenols
Biologically active compounds; Chemical compositions; Chemical variability; Intraspecific variability; Mucilage; Phenolic acids; Phenolic compounds; Tilia cordata
Engineering main heading: Essential oils
Species Index: Tilia cordata
ISSN: 09266690 CODEN: ICRDESource Type: Journal Original language: English
DOI: 10.1016/j.indcrop.2015.10.002Document Type: Article
Publisher: Elsevier