Volume 78, December 30, 2015, Pages 58-65
a
Laboratory of New Herbal
Products, Department of Vegetable and Medicinal Plants, Faculty of
Horticulture, Biotechnology and Landscape Architecture, Warsaw
University of Life Sciences SGGW, 159 Nowoursynowska Street, Warsaw,
Poland
b Division of Food Quality Evaluation, Department of Biotechnology and Microbiology, Faculty of Food Sciences, Warsaw University of Life Sciences SGGW, 159 Nowoursynowska Street, Warsaw, Poland
b Division of Food Quality Evaluation, Department of Biotechnology and Microbiology, Faculty of Food Sciences, Warsaw University of Life Sciences SGGW, 159 Nowoursynowska Street, Warsaw, Poland
Abstract
The aim of the study
was to investigate the chemical variability of small-leaved lime (Tilia
cordata Mill.) growing wild in the eastern area of Poland,
in terms of content and composition of phenolic compounds (phenolic
acids and flavonoids), composition of essential oil and content of
mucilage in flowers. Analyzed populations differed significantly when
regards the accumulation of biologically active compounds. The total
content of phenolic acids ranged from 0.17 to 1.87g×100g-1, flavonoids from 0.09 to 0.52g×100g-1, and mucilage from 0.6 to 3.0g×100g-1.
Using validated HPLC-DAD method, the chemical composition of phenolic
acids and flavonoids were determined. Among phenolic acids, four
compounds were identified with vanillic acid as the dominant one
(391.5-1718.0mg×100g-1). Among flavonoids, nine compounds
were found. Rutoside and (-)-epicatechin (tannin precursor) were present
in the highest amounts (1264.1-4654.6mg×100g-1 and 1000.3-4745.6mg×100g-1,
respectively). GC-MS and GC-FID were used to perform the qualitative
and quantitative analyze of the essential oil. The major compounds were
alkanes: tricosane (5.7-20.7%), pentacosane (3.0-6.1%) and heneicosane
(1.1-6.1%), as well as acetophenon (3.5-6.9%), nonanal (1.3-7.8%) and
nonanoic acid (3.9-18.2%). © 2015 Elsevier B.V.
Author keywords
Essential oil; Flavonoids; Intraspecific variability; Mucilage; Phenolic acids; Tilia cordata
Indexed keywords
Engineering controlled terms: Adhesives; Bioactivity; Chemical analysis; Flavonoids; Lime; Organic acids; Phenols
Biologically active compounds; Chemical
compositions; Chemical variability; Intraspecific variability; Mucilage;
Phenolic acids; Phenolic compounds; Tilia cordata
Engineering main heading: Essential oils
Species Index: Tilia cordata
ISSN: 09266690
CODEN: ICRDESource Type: Journal
Original language: English
DOI: 10.1016/j.indcrop.2015.10.002Document Type: Article
Publisher: Elsevier