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Phytochemistry, antioxidant capacity, total phenolic content and anti-inflammatory activity of Hibiscus sabdariffa leaves (Article)
a
New Use Agriculture and Natural Plant Products Program, Department
of Plant Biology and Pathology, Rutgers University, 59 Dudley Road, New
Brunswick, NJ, United States
b Department of Medicinal Chemistry, Rutgers University, Piscataway, NJ, United States
c Department of Pharmaceutics, Rutgers University, Piscataway, NJ, United States
b Department of Medicinal Chemistry, Rutgers University, Piscataway, NJ, United States
c Department of Pharmaceutics, Rutgers University, Piscataway, NJ, United States
Abstract
Abstract A liquid
chromatography-mass spectrometry method was developed for the
simultaneous separation, and determination of natural compounds
including phenolic acids and flavonoids in the leaves of Hibiscus
sabdariffa. By analyzing the UV and MS data, and comparison with
authenticated standards, 10 polyphenols including neochlorogenic acid,
chlorogenic acid, cryptochlorogenic acid, quercetin, kaempferol and
their glycosides were identified together with
5-(hydroxymethyl)furfural. Major constituents in the leaves of 25
different populations from worldwide accessions were quantified and
compared with each other. The total phenolic content of each accession
was determined using Folin-Ciocalteu assay, ranging from 18.98 ± 2.7 to
29.9 ± 0.5 mg GAE/g. Their in vitro antioxidant activities were measured
by ABTS radical cation decolorization assay, varying from 17.5 to 152.5
± 18.8 μmol Trolox/g. After the treatment of H. sabdariffa leaf
extract, the reduction of LPS-induced NO production dose-dependently in
RAW 264.7 cell indicates the extract's potential anti-inflammatory
activity. © 2015 Elsevier Ltd.
Author keywords
Anti-inflammatory; Antioxidant; Hibiscus sabdariffa; LC/MS; Leaf extract; Polyphenols
Indexed keywords
Engineering controlled terms: Chromatography; Flavonoids; Liquid chromatography; Mass spectrometry
Anti-inflammatories; Hibiscus sabdariffa; LC/MS; Leaf extracts; Polyphenols
Engineering main heading: Antioxidants
EMTREE drug terms: 5 (hydroxymethyl)furfural;
chlorogenic acid; cryptochlorogenic acid; glycoside; Hibiscus sabdariffa
extract; kaempferol; neochlorogenic acid; polyphenol; quercetin; trolox
C; unclassified drug
EMTREE medical terms: ABTS radical scavenging assay;
animal cell; antiinflammatory activity; antioxidant activity; Article;
controlled study; decolorization; dose response; drug determination; in
vitro study; mass spectrometry; mouse; nonhuman; phytochemistry; plant
leaf; quantitative analysis; ultraviolet radiation
Species Index: Hibiscus sabdariffa
Chemicals and CAS Registry Numbers: chlorogenic acid, 327-97-9; kaempferol, 520-18-3; polyphenol, 37331-26-3; quercetin, 117-39-5; trolox C, 56305-04-5
ISSN: 03088146
CODEN: FOCHDSource Type: Journal
Original language: English
DOI: 10.1016/j.foodchem.2015.06.006Document Type: Article
Publisher: Elsevier Ltd