Sunday, 21 February 2016

1797 February 21 Trinidad, West Indies surrenders to the British.

Born on February 21

1893 Andrés Segovia, Spanish classical guitarist.
1907 W.H. Auden, Pulitzer Prize-winning poet (The Age of Anxiety).
1927 Erma Bombeck, author and humorist (The Grass is Always Greener Over the Septic Tank).

Volume 190, 11 June 2016, Article number 17687, Pages 673-680

Phytochemistry, antioxidant capacity, total phenolic content and anti-inflammatory activity of Hibiscus sabdariffa leaves  (Article)

New Use Agriculture and Natural Plant Products Program, Department of Plant Biology and Pathology, Rutgers University, 59 Dudley Road, New Brunswick, NJ, United States
Department of Medicinal Chemistry, Rutgers University, Piscataway, NJ, United States
Department of Pharmaceutics, Rutgers University, Piscataway, NJ, United States


Abstract A liquid chromatography-mass spectrometry method was developed for the simultaneous separation, and determination of natural compounds including phenolic acids and flavonoids in the leaves of Hibiscus sabdariffa. By analyzing the UV and MS data, and comparison with authenticated standards, 10 polyphenols including neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, quercetin, kaempferol and their glycosides were identified together with 5-(hydroxymethyl)furfural. Major constituents in the leaves of 25 different populations from worldwide accessions were quantified and compared with each other. The total phenolic content of each accession was determined using Folin-Ciocalteu assay, ranging from 18.98 ± 2.7 to 29.9 ± 0.5 mg GAE/g. Their in vitro antioxidant activities were measured by ABTS radical cation decolorization assay, varying from 17.5 to 152.5 ± 18.8 μmol Trolox/g. After the treatment of H. sabdariffa leaf extract, the reduction of LPS-induced NO production dose-dependently in RAW 264.7 cell indicates the extract's potential anti-inflammatory activity. © 2015 Elsevier Ltd.

Author keywords

Anti-inflammatory; Antioxidant; Hibiscus sabdariffa; LC/MS; Leaf extract; Polyphenols

Indexed keywords

Engineering controlled terms: Chromatography; Flavonoids; Liquid chromatography; Mass spectrometry
Anti-inflammatories; Hibiscus sabdariffa; LC/MS; Leaf extracts; Polyphenols
Engineering main heading: Antioxidants
EMTREE drug terms: 5 (hydroxymethyl)furfural; chlorogenic acid; cryptochlorogenic acid; glycoside; Hibiscus sabdariffa extract; kaempferol; neochlorogenic acid; polyphenol; quercetin; trolox C; unclassified drug
EMTREE medical terms: ABTS radical scavenging assay; animal cell; antiinflammatory activity; antioxidant activity; Article; controlled study; decolorization; dose response; drug determination; in vitro study; mass spectrometry; mouse; nonhuman; phytochemistry; plant leaf; quantitative analysis; ultraviolet radiation
Species Index: Hibiscus sabdariffa
Chemicals and CAS Registry Numbers: chlorogenic acid, 327-97-9; kaempferol, 520-18-3; polyphenol, 37331-26-3; quercetin, 117-39-5; trolox C, 56305-04-5
ISSN: 03088146 CODEN: FOCHDSource Type: Journal Original language: English
DOI: 10.1016/j.foodchem.2015.06.006Document Type: Article
Publisher: Elsevier Ltd
  Simon, J.E.; New Use Agriculture and Natural Plant Products Program, Department of Plant Biology and Pathology, Rutgers University, 59 Dudley Road, United States
© Copyright 2015 Elsevier B.V., All rights reserved.