a
Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming, China
b College of Traditional Chinese Medicine, Yunnan University of Traditional Chinese Medicine, Kunming, China
c College of Resources and Environment, Yuxi Normal University, Yuxi, China
b College of Traditional Chinese Medicine, Yunnan University of Traditional Chinese Medicine, Kunming, China
c College of Resources and Environment, Yuxi Normal University, Yuxi, China
Abstract
Gentiana rigescens, an ethnomedicine,
is widely cultivated in Yunnan province of China. Although a wide range
of metabolites including iridoid glycosides, flavonoids and
triterpenoids have been reported in this ethnomedicine,
the data on accumulation and distribution of metabolites in certain
parts are limited. In this study, targeted metabolic fingerprinting of
iridoid glycosides based on liquid chromatography-ultraviolet
detection-tandem mass spectrometry (LC-UV-MS/MS) was developed to
investigate the metabolic similarities and differences in different
parts and origins. Thirty-one compounds, including iridoid glycosides
and flavonoids, were detected from targeted metabolite profiling and
plausibly assigned to the different parts of G. rigescens. Multivariate
statistical analysis was designed to reveal close chemical similarities
between all the selected samples and to identify key metabolites
characteristic of the standard. The results suggested that accumulation
and distribution of metabolites in aerial and underground parts were
different. Moreover, root samples tended to be grouped on the basis of
the geographical closeness of region. Five metabolites can be considered
as potential markers for the classification of underground parts from
different regions. These results provided chemical information on the
potential pharmaceutical value for further research, making G. rigescens
ideal for the rational usage of different parts and exploitation of the
source. © 2016 John Wiley & Sons, Ltd.
Author keywords
Chemical diversity; G. rigescens; Geographical origin; LC-MS/MS; Plant part
ISSN: 02693879
CODEN: BICHESource Type: Journal
Original language: English
DOI: 10.1002/bmc.3540Document Type: Article
Publisher: John Wiley and Sons Ltd
Funding Details
Number; Acronym; Sponsor: 81260608; NSFC; National Natural Science Foundation of China
