Volume 153, Issue 3, 14 May 2014, Pages 840–845
Research Paper
Bioactive compounds from Cornus officinalis fruits and their effects on diabetic nephropathy
Abstract
Ethnoparmacological relevance
The fruit of Cornus officinalis,
called “Shanzhuyu”, a traditional medicine in China, is used for the
treatment of kidney diseases, including diabetic nephropathy. The aim of
this study is to investigate the anti-diabetic nephropathy activity of
Shanzhuyu and the active compounds in the fruit.
Materials and methods
The air dried fruit of Cornus officinalis was extracted in 80% EtOH, the obtained residue was fractioned on D101 resin column eluted with H2O/EtOH
solution to get five crude fractions (fr. A–E). The anti-diabetic
nephropathy activity of fractions (fr. A–E) was evaluated in vitro
by inhibiting the expression of collagen IV (Col V), fibronectin (FN)
and IL-6 in high-glucose-induced mesangial cells. By preliminary
bio-assay screenings, repeated column chromatography on fraction B–D led
the isolation of 22 compounds, whose structures were determined by
extensive spectroscopic analysis, and the anti-diabetic nephropathy
activity of the isolated compounds was also evaluated.
Results
Two new iridoid glucosides, logmalicids A and B (1 and 2), together with 20 known compounds (3–22)
were isolated from the extract of Shanzhuyu under the bioassay-guided
screenings. The anti-diabetic nephropathy activity assay displayed that
fractions A, D and E could significantly inhibit the production of Col
IV; fractions A and C could significantly inhibit the expression of FN
and IL-6 in the high-glucose-stimulated mesangial cells at concentration
of 50 μg/mL; and loganin (3) and its derivatives (1 and 2) could significantly inhibit the expression of FN and IL-6 at concentration of 10 μM, respectively.
Conclusions
The results suggested that loganin and its derivatives were the active compounds in Cornus officinalis
fruit (Shanzhuyu) on diabetic nephropathy. This study further supported
the traditional use of Shanzhuyu to treat diabetic nephropathy and
related kidney diseases.
Graphical abstract
Chemical compounds studied in this article
- Loganin (PubChem CID: 87691);
- Loganic acid (PubChem CID: 89640);
- Cornuside (PubChem CID: 131348);
- Secoxyloganin (PubChem CID: 162868);
- 7-α-morroniside (PubChem CID: 21593530);
- Caffeic acid (PubChem CID: 689043);
- Tachioside (PubChem CID: 11962143);
- Kaempferol-3-O-β-d-rutinoside (PubChem CID: 12313332);
- Quercetin (PubChem CID: 5280343)
Keywords
- Cornus officinalis fruit;
- Iridoid glucoside;
- Loganin;
- Diabetic nephropathy
- Corresponding author. Tel./fax: +86 871 5223048.
- Corresponding author. Tel./fax: +86 551 65161115.
- 1
- These authors contributed equally.
