PLoS One. 2015 Nov 4;10(11):e0141475. doi: 10.1371/journal.pone.0141475. eCollection 2015.
Piscine Reovirus: Genomic and Molecular Phylogenetic Analysis from Farmed and Wild Salmonids Collected on the Canada/US Pacific Coast.
Siah A1, Morrison DB2, Fringuelli E3, Savage P3, Richmond Z1, Johns R1, Purcell MK4, Johnson SC5, Saksida SM1.
Abstract
Piscine
reovirus (PRV) is a double stranded non-enveloped RNA virus detected in
farmed and wild salmonids. This study examined the phylogenetic
relationships among different PRV sequence types present in samples from
salmonids in Western Canada and the US, including Alaska (US), British Columbia
(Canada) and Washington State (US). Tissues testing positive for PRV
were partially sequenced for segment S1, producing 71 sequences that
grouped into 10 unique sequence types. Sequence analysis revealed no
identifiable geographical or temporal variation among the sequence
types. Identical sequence types were found in fish sampled in 2001, 2005
and 2014. In addition, PRV positive samples from fish derived from
Alaska, British Columbia
and Washington State share identical sequence types. Comparative
analysis of the phylogenetic tree indicated that Canada/US Pacific
Northwest sequences formed a subgroup with some Norwegian sequence types
(group II), distinct from other Norwegian and Chilean sequences (groups
I, III and IV). Representative PRV positive samples from farmed and
wild fish in British Columbia
and Washington State were subjected to genome sequencing using next
generation sequencing methods. Individual analysis of each of the 10
partial segments indicated that the Canadian and US PRV sequence types
clustered separately from available whole genome sequences of some
Norwegian and Chilean sequences for all segments except the segment S4.
In summary, PRV was genetically homogenous over a large geographic
distance (Alaska to Washington State), and the sequence types were
relatively stable over a 13 year period.
