J Chromatogr A. 2015 Oct 9. pii: S0021-9673(15)01454-5. doi: 10.1016/j.chroma.2015.10.010. [Epub ahead of print]
Abstract
Two tansy (Tanacetum vulgare
L.) essential oils were obtained by steam distillation of the capitula
with subsequent liquid-liquid extraction (oil 1) or with use of an
auxiliary phase for the trapping of the steam components (oil 2). These
oils were investigated against Bacillus subtilis F1276, B. subtilis
spizizenii (DSM 618), Xanthomonas euvesicatoria, Pseudomonas syringae
pv. maculicola, Ralstonia solanacearum strain GMI1000 and Aliivibrio
fischeri, using the coupling of high-performance thin-layer
chromatography to direct bioautography (HPTLC-DB). Using this method
with the potato and tomato pathogen R. solanacearum is shown for the
first time. Due to the advanced extraction process, oil 2 was richer in
components and provided more inhibition zones. The main bioactive
components were identified by scanning HPTLC-Direct Analysis in Real
Time mass spectrometry (HPTLC-DART-MS) and solid-phase microextraction
gas chromatography electron impact MS (SPME-GC-EI-MS) as cis- and
trans-chrysanthenol as well as trans-chrysanthenyl acetate.
cis-Chrysanthenol exhibited antibacterial effects against all tested
bacteria, whereas trans-chrysanthenol inhibited B. subtilis, R.
solanacearum and A. fischeri. trans-Chrysanthenyl acetate was an
inhibitor for X. euvesicatoria, R. solanacearum and A. fischeri.
Although HPTLC-DART-MS resulted in a comparable fragmentation, the
ionization characteristics and the recorded mass spectra clearly showed
that DART is a softer ionization technique than EI. It is also more
affected by ambient conditions and thus prone to additional oxidation
products.
Copyright © 2015 Elsevier B.V. All rights reserved.
Copyright © 2015 Elsevier B.V. All rights reserved.
KEYWORDS:
Chrysanthenol; Chrysanthenyl acetate; HPTLC-DART-MS; HPTLC-direct bioautography; SPME-GC-EI-MS; Tansy (Tanacetum vulgare L.) essential oil- PMID:
- 26499972
- [PubMed - as supplied by publisher]
