a
Department of Pharmaceutical Botany, Jagiellonian University, Collegium Medicum, Medyczna St 9, Kraków, Poland
b Department of Inorganic and Analytical Chemistry, Jagiellonian University, Collegium Medicum, Medyczna St 9, Kraków,
b Department of Inorganic and Analytical Chemistry, Jagiellonian University, Collegium Medicum, Medyczna St 9, Kraków,
Abstract
The popularity of
Agaricus bisporus results not only from the quality of the flavors, but
also from the content of many substances of therapeutic properties. This
paper presents a study on RP-HPLC determination of the content of
indole compounds released from the lyophilized biomass from in vitro
cultures of A. bisporus into artificial digestive juices at 37 °C. A.
bisporus in vitro cultures were grown on media enriched with zinc salts.
The release of 5-hydroxy-l-tryptophan and l-tryptophan was found in the
greatest number of samples. The content of 5-hydroxy-l-tryptophan in
the investigated samples ranged from 86.62 to 531 mg/100 g d.w. The
amount of l-tryptophan was determined within the range of 1.63-4.68
mg/100 g d.w. and for melatonin 0.43-0.64 mg/100 g d.w. It was
demonstrated for the first time that in vitro cultures of A. bisporus
release indole compounds in conditions simulating the human digestive
tract. © 2015 Elsevier Ltd. All rights reserved.
Author keywords
Agaricus bisporus; Artificial gastric juices; In vitro culture; Indole compounds; Zinc
Indexed keywords
Engineering controlled terms: Amino acids; Fungi; Salts; Zinc
Agaricus bisporus; Gastric juice; Human digestive
tract; In-vitro; Indole compounds; L-tryptophan; Number of samples;
Therapeutic properties
Engineering main heading: Polycyclic aromatic hydrocarbons
ISSN: 03088146
CODEN: FOCHDSource Type: Journal
Original language: English
DOI: 10.1016/j.foodchem.2015.12.041Document Type: Arti
