(Article)
a Department of Natural Science and Quality Assurance, Faculty of Commodity Science, Poznań University of Economics, Al. Niepodległości 10, Poland
b Department of Pathogen Genetics and Resistance, Institute of Genetics of the Polish Academy of Sciences, 34 Strzeszyńska,
b Department of Pathogen Genetics and Resistance, Institute of Genetics of the Polish Academy of Sciences, 34 Strzeszyńska,
Abstract
Triterpene saponins are important bioactive constituents with an enormous variety in structure widely distributed in manyplants. Here, we profiled triterpene saponins from the skin and flesh of red beetroot Beta vulgaris L. cultivars Nochowski from 2012 and 2013 season using reversed-phase liquid chromatography combined with negative-ion electrospray ionisation quadrupole mass spectrometry. We tentatively identified 44 triterpene saponins, of which 37 had not been detected previously in the root of red beets and 27 saponins were tentatively identified as potentially new compounds. All observed compounds were glycosides of four different aglycone structures, of which akebonoic acid and gypsogenin were not detected previous in red beetroot. Based on the high-resolution mass measurements among these 44 detected saponins 10 groups of isomers were identified. We report for the first time that 18 saponins with dioxolane-type (2 saponins) and acetal-type (16 saponins) substituents were detected in the roots of red beet. © 2015 Elsevier Ltd. All rights reserved.
Author keywords
Beta vulgaris L.; HPLC-MS; Red beet; Triterpene saponins
Indexed keywords
Engineering controlled terms: Electrospray ionization; Ionization of liquids; Isomers; Liquid chromatography; Mass spectrometry; Negative ions; Plants (botany)
Beta vulgaris L; High resolution; HPLC-MS; Mass measurements; Quadrupole mass spectrometry; Red beet; Reversed phase liquid-chromatography; Triterpene saponins
Engineering main heading: Metabolites
EMTREE drug terms: saponin derivative; triterpene saponin; unclassified drug
EMTREE medical terms: Article; beetroot; chemical structure; high performance liquid chromatography; high performance liquid chromatography and electrospray ionisation ion trap mass spectrometry; high resolution ultra high performance liquid chromatography and heated electrospray ionisation quadrupole orbitrap mass spectrometry; isomer; molecular weight; tandem mass spectrometry
Species Index: Beta vulgaris; Beta vulgaris subsp. vulgaris
ISSN: 03088146 CODEN: FOCHDSource Type: Journal Original language: English
DOI: 10.1016/j.foodchem.2015.07.111Document Type: Article
Publisher: Elsevier Ltd
