a
Bioactive Molecules Research Group, Faculty of Sciences, Doctoral
School of Sciences and Technologies, Lebanese University, B.P. 90656,
Jdaidet el-Matn, Lebanon
b Laboratoire d'Automatique et de Génie des Procédés (LAGEP), UMR-CNRS 5007, Université Claude Bernard Lyon 1, CPE Lyon, Bat 308G, 43 Boulevard du 11 Novembre 1918, Villeurbanne Cedex, France
b Laboratoire d'Automatique et de Génie des Procédés (LAGEP), UMR-CNRS 5007, Université Claude Bernard Lyon 1, CPE Lyon, Bat 308G, 43 Boulevard du 11 Novembre 1918, Villeurbanne Cedex, France
Abstract
This work is
dedicated to prepare liposomal dry powder formulations of inclusion
complexes of clove essential oil (CEO) and its main component eugenol
(Eug). Ethanol injection method and membrane contactor were applied to
prepare liposomes at laboratory and large scale, respectively. Various
liposomal formulations were tested: (1) free
hydroxypropyl-β-cyclodextrin loaded liposomes; (2) drug in
hydroxypropyl-β-cyclodextrin in liposomes (DCL); (3) DCL2
obtained by double loading technique, where the drug is added in the
organic phase and the inclusion complex in the aqueous phase. Liposomes
were characterized for their particle size, polydispersity index, Zeta
potential, morphology, encapsulation efficiency of CEO components and
Eug loading rate. Reproducible results were obtained with both injection
devices. Compared to Eug-loaded liposomes, DCL and DCL2 improved the loading rate of Eug and possessed smaller vesicles size. The DPPH• scavenging activity of Eug and CEO was maintained upon incorporation of Eug and CEO into DCL and DCL2. Contrary to DCL2,
DCL formulations were stable after 1 month of storage at 4 °C and upon
reconstitution of the dried lyophilized cakes. Hence, DCL in aqueous and
lyophilized forms, are considered as a promising carrier system to
preserve volatile and hydrophobic drugs enlarging their application in
cosmetic, pharmaceutical and food industries. © 2015 Elsevier Ltd. All
rights reserved.
Author keywords
Clove essential oil; Double loading technique; Drug in cyclodextrin in liposomes; Eugenol; Freeze-drying; Scale-up
Indexed keywords
Engineering controlled terms: Cyclodextrins; Encapsulation; Essential oils; Oils and fats; Particle size
Encapsulation efficiency; Eugenol; Freeze drying;
Liposomal formulation; Loading technique; Polydispersity indices;
Scale-up; Scavenging activities
Engineering main heading: Liposomes
