Thursday, 29 December 2016

Laserpitium zernyi Hayek Flower and Herb Extracts: Phenolic Compounds, and Anti-edematous, Antioxidant and Antimicrobial Activities

2016 Dec 16. doi: 10.1002/cbdv.201600432. [Epub ahead of print]

Popović VB1,2, Tomić MA3, Stepanović-Petrović RM3, Micov AM3, Milenković MT4, Petrović SD1, Ušjak LJ1, Niketić MS5.

Author information

  • 1University of Belgrade - Faculty of Pharmacy, Department of Pharmacognosy, V. Stepe 450, 11221, Belgrade, Serbia.
  • 2Receptor Research Laboratories, Nuclear Receptor Lab, VIB Medical Biotechnology Center, VIB, Ghent University, Albert Baertsoenkaai 3, B-9000, Ghent, Belgium.
  • 3University of Belgrade - Faculty of Pharmacy, Department of Pharmacology, V. Stepe 450, 11221, Belgrade, Serbia.
  • 4University of Belgrade - Faculty of Pharmacy, Department of Microbiology and Immunology, V. Stepe 450, 11221, Belgrade, Serbia.
  • 5Natural History Museum, Njegoševa 51, 11000, Belgrade, Serbia.


Phenolic compounds and different biological activities of the dry methanol extracts of the flowers and the herb (aerial parts without flowers) of Laserpitium zernyi Hayek (Apiaceae) were investigated. The total phenolic contents in the extracts were determined spectrophotometrically using Folin-Ciocalteu reagent. In both extracts, apigenin, luteolin, their 7-O-glucosides and chlorogenic acid were detected by HPLC. Identified phenolics were quantified in both extracts, except luteolin in L. zernyi herb extract. The extracts (p.o.) were tested for anti-edematous activity in a model of carrageenan ( induced rat paw edema. Antioxidant activity of the extracts was assessed by FRAP assay and DPPH and 'OH radicals scavenging tests. Antimicrobial activity was investigated using broth microdilution test against five Gram-positive and three Gram-negative bacteria, as well as against two strains of Candida albicans. The polyphenol-richer flower extract exerted higher anti-edematous and antioxidant activities. The herb extract exhibited better antimicrobial effect against Micrococcus luteus, Enterococcus faecalis, Bacillus subtilis and Pseudomonas aeruginosa, while against other tested microorganisms the activity of both extracts was identical. Demonstrated biological activities of L. zernyi flower and herb extracts represent a good basis for their further investigation as potential new herbal medicinal raw materials. This article is protected by copyright. All rights reserved.


Anti-edematous activity; Antimicrobial activity; Antioxidant activity; Laserpitium zernyi aerial parts; Polyphenols