- 1
- Department
of General Biochemistry, Faculty of Biology and Environmental
Protection, University of Lodz, Pomorska 141/143, 90-236 Lodz, Poland.
- 2
- Department
of Molecular Genetics, Faculty of Biology and Environmental Protection,
University of Lodz, Pomorska 141/143, 90-236 Lodz, Poland.
- 3
- Chemistry Department, Institute of Applied Radiation Chemistry, Lodz University of Technology, Wroblewskiego 15, 93-590 Lodz, Poland.
- 4
- Division
of Organic and Pharmaceutical Technology, Faculty of Chemistry, Wroclaw
University of Science and Technology, Wybrzeze Wyspianskiego 29, 50-370
Wroclaw, Poland.
- 5
- Department
of General Biochemistry, Faculty of Biology and Environmental
Protection, University of Lodz, Pomorska 141/143, 90-236 Lodz, Poland. Electronic address: zbikow@biol.uni.lodz.pl.
Abstract
Radioprotective
potential of the polyphenolic glycoconjugates, isolated from flowers of
Sanguisorba officinalis L. (So) and Erigeron canadensis L. (Ec), and
from leaves of Fragaria vesca L. (Fv) and Rubus plicatus Whe. Et N. E.
(Rp) as well as their aglycones (SoA, EcA, FvA and RpA, respectively),
against γ-radiation-induced lipid peroxidation in human plasma and DNA
damage in lymphocytes, were investigated in vitro. These properties were
assessed by measuring the concentration of thiobarbituric acid reactive
substances (TBARS) and using the alkaline comet assay, and were
compared to the protective effects of rutin (R) and quercetin (Q).
Cytotoxicity of the glycoconjugates/aglycones towards L929 mouse
fibroblasts and human lymphocytes were also measured. Plant products
from S. officinalis, similar to Q, were able to reduce the most
radiation-induced lipid peroxidation as well as DNA damage and extent of
oxidative damage to the DNA basis. Contrary to the pure flavonoids,
where Q was shown to be significantly more effective than its glycoside
R, the results did not show more benefit with application of SoA/EcA
over So/Ec in terms of lipid peroxidation inhibition. Moreover,
glycoconjugates Ec and So showed much higher capacity in protecting
lymphocytes against radiation-induced genotoxicity which may suggest
that between the polyphenolic and polysaccharide parts exist some
synergistic effects. There were no significant differences between Fv
versus FvA or Rp versus RpA in terms of the provided radioprotection.
Summarizing, plant glycoconjugates isolated by the multi-step method
offered sufficient radioprotection. In addition, they possess many
advantages, compared to the synthetic polyphenolic compounds or the
plant extracts, such as water-solubility and minor toxicity.
Copyright © 2017 Elsevier B.V. All rights reserved.
KEYWORDS:
Aglycone; Erigeron canadensis; Polyphenolic glycoconjugate; Radioprotector; Sanguisorba officinalis; γ-Irradiation
