Nat Prod Res. 2017 Apr;31(7):802-809. doi: 10.1080/14786419.2016.1247077. Epub 2016 Dec 15.

Chemical profiling of the tuber of Stephania cambodica Gagnep. (Menispermaceae) and analytical control by UHPLC-DAD.

Dary C^1,2,3, Bun SS¹, Herbette G⁴, Mabrouki F¹, Bun H⁵, Kim S⁶, Jabbour F³, Hul S³, Baghdikian B¹, Ollivier E¹.

Author information

1

a Laboratory of Pharmacognosy and Ethnopharmacology, Faculty of Pharmacy , Aix Marseille Univ, UMR-MD3 , Marseille , France.

2

b Research Unit , University of Health Sciences , Phnom Penh , Cambodia.

3

c Muséum national d'Histoire naturelle, Institut de Systématique, Évolution, Biodiversité, UMR 7205 ISYEB MNHN/CNRS/UPMC/EPHE , Sorbonne Université , Paris , France.

4

d Spectropole, Service 511, Aix Marseille Univ , Marseille , France.

5

e Laboratory of Pharmacokinetics and Toxicokinetics, Faculty of Pharmacy , Aix Marseille Univ , Marseille , France.

6

f Laboratory of Phytochemistry, Faculty of Pharmacy , University of Health Sciences , Phnom Penh , Cambodia.

Abstract

A new aporphine glycoside (1), named 'angkorwatine', and eight known alkaloids: oblongine (2), stepharine (3), asimilobine-β-d-glucopyranoside (4), isocorydine (5), tetrahydropalmatine (THP) (6), jatrorrhizine (7), palmatine (PAL) (8), and roemerine (ROE) (9) were simultaneously isolated from the tuber of Stephania cambodica. The development and validation of UHPLC-DAD method was carried out for the quantification of marker compounds (PAL, ROE, THP) of S. cambodica. In addition to good selectivity and linearity (r² > 0.997), trueness, precision, and accuracy of the method did not exceed the acceptance limit of ±10% for ROE, THP and ±20% for PAL. Consequently, this method is able to provide accurate results between 1.39-4.18 μg/mL, 2.01-30.72 μg/mL, and 4.29-64.42 μg/mL for PAL, ROE, and THP, respectively. This study shows that the validated UHPLC method is a rapid, innovative and effective analytical approach to control quality of tubers of S. cambodica and to regulate the usage of this plant in traditional medicine.

KEYWORDS:

Accuracy profile; alkaloid; method validation; palmatine; quantification; roemerine; tetrahydropalmatine