Bacterial cellulose-lignin composite hydrogel as a promising agent in chronic wound healing.

nt J Biol Macromol. 2018 Oct 15;118(Pt A):494-503. doi: 10.1016/j.ijbiomac.2018.06.067. Epub 2018 Jun 15. Zmejkoski D1, Spasojević D2, Orlovska I3, Kozyrovska N3, Soković M4, Glamočlija J5, Dmitrović S6, Matović B7, Tasić N8, Maksimović V9, Sosnin M10, Radotić K11. Author information 1 Vinča Institute of Nuclear Sciences, University of Belgrade, Mihaila Petrovića Alasa 12-14, 11001 Belgrade, Serbia. Electronic address: danica@vinca.rs. 2 Institute for Multidisciplinary Research, University of Belgrade, Kneza Višeslava 1, 11000 Belgrade, Serbia. Electronic address: dragica@imsi.rs. 3 Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Zabolotnogo Str. 150, Kyiv, Ukraine. 4 Institute for Biological Research 'Siniša Stanković', Mycological Laboratory, Department of Plant Physiology, University of Belgrade, Bulevar despota Stefana 142, 11000 Belgrade, Serbia. Electronic address: mris@ibiss.bg.ac.rs. 5 Institute for Biological Research 'Siniša Stanković', Mycological Laboratory, Department of Plant Physiology, University of Belgrade, Bulevar despota Stefana 142, 11000 Belgrade, Serbia. Electronic address: jasna@ibiss.bg.ac.rs. 6 Vinča Institute of Nuclear Sciences, University of Belgrade, Mihaila Petrovića Alasa 12-14, 11001 Belgrade, Serbia. 7 Vinča Institute of Nuclear Sciences, University of Belgrade, Mihaila Petrovića Alasa 12-14, 11001 Belgrade, Serbia. Electronic address: mato@vinca.rs. 8 Institute for Multidisciplinary Research, University of Belgrade, Kneza Višeslava 1, 11000 Belgrade, Serbia. Electronic address: nikola.tasic@imsi.bg.ac.rs. 9 Institute for Multidisciplinary Research, University of Belgrade, Kneza Višeslava 1, 11000 Belgrade, Serbia. Electronic address: maxivuk@imsi.rs. 10 Institute of Physics, National Academy of Sciences of Ukraine, 46 Nauki Ave., 03028 Kyiv, Ukraine. Electronic address: lukh@iop.kiev.ua. 11 Institute for Multidisciplinary Research, University of Belgrade, Kneza Višeslava 1, 11000 Belgrade, Serbia. Electronic address: xenia@imsi.rs. Abstract Lignins and lignin-derived compounds are known to have antibacterial properties. The wound healing agents in the form of dressings produce faster skin repair and decrease pain in patients. In order to create an efficient antimicrobial agent in the form of dressing in the treatment of chronic wounds, a composite hydrogel of bacterial cellulose (BC) and dehydrogenative polymer of coniferyl alcohol (DHP), BC-DHP, was designed. Novel composite showed inhibitory or bactericidal effects against selected pathogenic bacteria, including clinically isolated ones. The highest release rate of DHP was in the first hour, while after 24 h there was still slow release of small amounts of DHP from BC-DHP during 72 h monitoring. High-performance liquid chromatography coupled with mass-spectrometry showed that BC-DHP releases DHP oligomers, which are proposed to be antimicrobially active DHP fractions. Scanning electron microscopy and atomic force microscopy micrographs proved a dose-dependent interaction of DHP with BC, which resulted in a decrease of the pore number and size in the cellulose membrane. The Fourier-transform infrared absorption spectra of the BC-DHP showed that DHP was partly bound to the BC matrix. The swelling and crystallinity degree were dose-dependent. All obtained results confirmed BC-DHP composite as a promising hydrogel for wounds healing. KEYWORDS: Antimicrobial activity; Bacterial cellulose; Lignin model polymer PMID: 29909035 DOI: 10.1016/j.ijbiomac.2018.06.067 Share on FacebookShare on TwitterShare on Google+