Volume 190, Issues 1–2, 23 November 2012, Pages 204–209
Rotenoid content and in vitro acaricidal activity of Tephrosia vogelii leaf extract on the tick Rhipicephalus appendiculatus
Abstract
This study aimed to determine the rotenoid content of leaf extracts of the white (TVW) and purple (TVP) varieties of Tephrosia vogelii, both collected in North-Kivu Province, Democratic Republic of Congo and to evaluate their in vitro acaricidal efficacy on the tick Rhipicephalus appendiculatus.
The high performance liquid chromatography analysis of rotenoid
compounds from those extracts revealed that the contents of rotenone and
deguelin were respectively higher in the leaves of TVW (0.044% and
1.13%) than in TVP (0.014% and 0.66%). Batches of 20 live adult ticks
were immersed for 15 min in six different doses of each plant extract
(0.625; 1.25; 2.5; 5; 10 and 20 mg/mL of distilled water) and in the
solution of Milbitraz® (12.5% m/v emulsifiable concentrate of
amitraz) as a positive control. Additionally 9.5% ethanol and distilled
water control groups were included. Tick mortalities were recorded
every 24 h for 5 days. The results indicated that there was no
significant difference (P > 0.05) between the acaricidal effect of Milbitraz®
and the plant material used at a dose of at least 2.5 or 5 mg/mL for
TVW and TVP respectively. However, the dose response relationship
determined at the fifth day after treatment showed a similar acaricidal
effect for the two plant varieties with similar lethal dose 50 (LD50) of 0.83 and 0.81 mg/mL for TVW and TVP respectively. It is concluded that T. vogelii leaves may be used for the control of R. appendiculatus in areas where synthetic acaricides are either not available or affordable. However, T. vogelii extract should be sprayed in order to limit the potential risks of ecotoxicity linked to rotenoid compounds.
Keywords
- Tephrosia vogelii (Fabaceae);
- Rotenone;
- Deguelin;
- LD50;
- Rhipicephalus appendiculatus
- Corresponding author. Tel.:
+32 0 4 366 45 79; fax: +32 0 4 366 42 61.
Copyright © 2012 Elsevier B.V. All rights reserved.
