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Thursday, 20 August 2015

Trypanocidal efficacy of two indigeneous ethanolic plant extracts (Mimosa pigra and Ipomoea asarifolia) against Trypanosoma evansi phospholipase A2 activity

Journal of Acute Disease
Volume 4, Issue 1, March 2015, Pages 28–31
Open Access
Document heading

Trypanocidal efficacy of two indigeneous ethanolic plant extracts (Mimosa pigra and Ipomoea asarifolia) against Trypanosoma evansi phospholipase A2 activity


doi:10.1016/S2221-6189(14)60078-0
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Open Access funded by Hainan Medical College
Under a Creative Commons license

Abstract

Objective

To study the inhibitory activity of ethanolic extract from Mimosa pigra and Ipomoea asarifolia against Trypanosoma evansi (T. evansi) calcium dependent phospholipase A2.

Methods

The calcium dependent phospholipase A2 (E C 3.1.1.4) enzyme was isolated from T. evansi and purified to electrophoretic homogeneity under non denaturing conditions. It was solubilized from T. evansi cells recovered from white albino rats which were previously inoculated by intraperitoneal injection of infected camel blood. Two indigeneous ethanolic plant extracts used locally for treatment of trypanosomiasis were tested for the inhibition of phospholipases A2.

Results

Double reciprocal plots of the initial velocity data of the inhibition by the indigenous plant extracts revealed a noncompetitive pattern of inhibition for the Ipomoea asarifolia and a competitive inhibition for Mimosa pigra in a dose dependent fashion. The extrapolated inhibition binding constant (Ki) of these extracts were found to be 2.0×102 μg/mL and 1.12×102 μg/mL respectively.

Conclusions

The low Ki values obtained for these extracts towards this enzyme are an indication of high affinity of the extract or the active components (present in the plants) are for these enzyme and therefore, could be explored to serve as a cheap source of T. evansi PLA2 antidote and as well help in designing a novel drug with high efficiency.

Keywords

  • Phospholipase A2;
  • Indigeneous plant extract;
  • Trypanosomiasis
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Available online 26 Jan 2015

Corresponding author: Abdulkadir Abdullahi, Federal University of technology Minna, Nigeria
Copyright © 2015 Hainan Medical College. E-edition published by Elsevier (Singapore) Pte Ltd. Published by Elsevier (Singapore) Pte Ltd.
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