Journal of Herbal Medicine

Volume 5, Issue 2, June 2015, Pages 77–85

Original Research Article

Health attributes of an endemic orchid from Eastern Anatolia, Dactylorhiza chuhensis Renz&Taub. – In vitro investigations

• Abdullah Dalar^{a, b},
• Yu Guo^{a, c},
• Nevzat Esim^d,
• Aydin Sukru Bengu^e,
• Izabela Konczak^{a, f, g, ,}

doi:10.1016/j.hermed.2015.02.001

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Highlights

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Dactylorhiza chuhensis leaf extract has moderate enzyme inhibitory activities.

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D. chuhensis leaf extract exhibits anti-inflammatory properties.

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The leaf of D. chuhensis can serve as a source of physiologically active phytochemicals.

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Abstract

Phytochemical composition and potential health attributes of Dactylorhiza chuhensis Renz&Taub., an endemic orchid from Eastern Anatolia, were investigated. Lyophilized methanol-based extracts obtained from leaf, flower, stem and tuber were investigated for the presence of phenolic compounds [Folin–Ciocalteu assay and high performance liquid chromatography analysis (HPLC)], antioxidant capacities [ferric reducing antioxidant power (FRAP) and oxygen radical absorbance capacity (ORAC) assay] and enzyme-inhibitory activities [lipase, α-amylase, α-glucosidase and angiotensin converting enzyme (ACE)]. The tuber, used as a traditional remedy and utilized by the pharmaceutical industry, had the lowest content of total phenolics, inferior antioxidant and enzyme-inhibitory activities. The highest phenolic content (44.9 ± 0.8 mg GAE/g DW) was exhibited by the leaf extract, which also showed superior reducing (736.8 ± 16.2 μmol Fe²⁺/g DW) and oxygen radical scavenging capacities (2715.8 ± 83.5 μmol Trolox E/g DW). The inhibitory activities of the leaf extract toward α-amylase, α-glucosidase and ACE were moderate. Applied at non-toxic concentrations, the leaf extract effectively reduced accumulation of nitric oxide (NO) in lipopolysaccharide (LPS)-activated hepatocellular carcinoma (HepG2) cells. Further studies towards potential utilization of D. chuhensis leaf as a source of physiologically active phytochemcials are justified.

Keywords

• Dactylorhiza chuhensis Renz&Taub.;
• Antioxidant;
• Enzyme-inhibitory activity;
• Total phenolics

Corresponding author at: Food Science and Technology, School of Chemical Sciences and Engineering, The University of New South Wales, Sydney, NSW 2052, Australia. Tel.: +61 2 98681436.

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